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IPR018338 is a Carbonic anhydrase, alpha-class, conserved site.
<p>Carbonic anhydrases (CA: [ec:4.2.1.1]) are zinc metalloenzymes which catalyse the reversible hydration of carbon dioxide to bicarbonate [[cite:PUB00044806], [cite:PUB00044807]]. CAs have essential roles in facilitating the transport of carbon dioxide and protons in the intracellular space, across biological membranes and in the layers of the extracellular space; they are also involved in many other processes, from respiration and photosynthesis in eukaryotes to cyanate degradation in prokaryotes. There are five known evolutionarily distinct CA families (alpha, beta, gamma, delta and epsilon) that have no significant sequence identity and have structurally distinct overall folds. Some CAs are membrane-bound, while others act in the cytosol; there are several related proteins that lack enzymatic activity. The active site of alpha-CAs is well described, consisting of a zinc ion coordinated through 3 histidine residues and a water molecule/hydroxide ion that acts as a potent nucleophile. The enzyme employs a two-step mechanism: in the first step, there is a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide; in the second step, the active site is regenerated by the ionisation of the zinc-bound water molecule and the removal of a proton from the active site [[cite:PUB00017891]]. Beta-and gamma-CAs also employ a zinc hydroxide mechanism, although at least some beta-class enzymes do not have water directly coordinated to the metal ion.</p> <p>This entry represents a conserved site based around one of the zinc-binding histidines in the alpha class of carbonic anhydrases.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Carbonate dehydratase activity | Catalysis of the reaction: H2CO3 = CO2 + H2O. | ||
| Molecular function | Zinc ion binding | Interacting selectively and non-covalently with zinc (Zn) ions. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | A-type carbonic anhydrase [Lotus japonicus] gi|152955979|emb|CAM59682.1| | 18 | ||
| – | A-type carbonic anhydrase [Lotus japonicus] gi|152955979|emb|CAM59682.1| | 21 | ||
| – | A-type carbonic anhydrase [Lotus japonicus] gi|152955979|emb|CAM59682.1| | 12 | ||
| – | PREDICTED: bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like [Glycine max] gi|356533269|ref|XP_003535188.1| | 21 | ||
| – | PREDICTED: bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like [Glycine max] gi|356536979|ref|XP_003537009.1| | 19 | ||
| – | Carbonic anhydrase family protein; TAIR: AT4G20990.1 alpha carbonic anhydrase 4; Swiss-Prot: sp|F4JIK2|ATCA4_ARATH Alpha carbonic anhydrase 4; TrEMBL-Plants: tr|A7DX12|A7DX12_LOTJA A-type carbonic anhydrase; Found in the gene: LotjaGi1g1v0670000 | 22 | ||
| – | Carbonic anhydrase family protein; TAIR: AT4G20990.1 alpha carbonic anhydrase 4; Swiss-Prot: sp|F4JIK2|ATCA4_ARATH Alpha carbonic anhydrase 4; TrEMBL-Plants: tr|A7DX12|A7DX12_LOTJA A-type carbonic anhydrase; Found in the gene: LotjaGi1g1v0670000 | 14 | ||
| – | Carbonic anhydrase, putative; TAIR: AT1G08080.1 alpha carbonic anhydrase 7; Swiss-Prot: sp|Q8L817|ATCA7_ARATH Alpha carbonic anhydrase 7; TrEMBL-Plants: tr|I3S826|I3S826_LOTJA Uncharacterized protein; Found in the gene: LotjaGi5g1v0083400 | 23 | ||
| – | Carbonic anhydrase, putative; TAIR: AT1G08080.1 alpha carbonic anhydrase 7; Swiss-Prot: sp|Q8L817|ATCA7_ARATH Alpha carbonic anhydrase 7; TrEMBL-Plants: tr|A0A072VKK0|A0A072VKK0_MEDTR Eukaryotic-type carbonic anhydrase; Found in the gene: LotjaGi5g1v0084500 | 22 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 11.11 |