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Field | Value |
---|---|
Namespace | Molecular function |
Short description | DNA photolyase activity |
Full defintion | Catalysis of the repair of a photoproduct resulting from ultraviolet irradiation of two adjacent pyrimidine residues in DNA. |
Subterm of |
The relationship of GO:0003913 with other GO terms.
Relationship type | GO terms |
---|---|
Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0003913, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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GOC | mah |
PMID | Role of two histidines in the (6-4) photolyase reaction. J Biol Chem. 2001 Mar 30; 276 (13): 10103–9.PMID: 11124949 The reaction mechanism of Xenopus (6-4) photolyase was investigated using several mutant enzymes. In the active site, which is homologous between the cis,syn-cyclobutane pyrimidine dimer and (6-4) photolyases, four amino acid residues that are specific to (6-4) photolyase, Gln(288), His(354), Leu(355), and His(358), and two conserved tryptophans, Trp(291) and Trp(398), were substituted with alanine. Only the L355A mutant had a lower affinity for the substrate, which suggested a hydrophobic interaction with the (6-4) photoproduct. Both the H354A and H358A mutations resulted in an almost complete loss of the repair activity, although the Trp(291) and Trp(398) mutants retained some activity. Taking the pH profile of the (6-4) photolyase reaction into consideration with this observation, we propose a mechanism in which these histidines catalyze the formation of the four-membered ring intermediate in the repair process of this enzyme. When deuterium oxide was used as a solvent, the repair activity was decreased. The proton transfer shown by this isotope effect supports the proposed mechanism. The substrate binding and the reaction mechanism are discussed in detail using a molecular model. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | Cryptochrome DASH [Medicago truncatula] gi|357466479|ref|XP_003603524.1| | 2 | ||
– | PREDICTED: cryptochrome DASH, chloroplastic/mitochondrial-like [Cicer arietinum] gi|502132182|ref|XP_004501253.1| | 2 | ||
– | Cryptochrome; TAIR: AT5G24850.1 cryptochrome 3; Swiss-Prot: sp|Q84KJ5|CRYD_ARATH Cryptochrome DASH, chloroplastic/mitochondrial; TrEMBL-Plants: tr|G7JCC1|G7JCC1_MEDTR Cryptochrome, DASH family protein; Found in the gene: LotjaGi1g1v0392600 | 2 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Biological process | DNA repair | 1 | 33.33 |