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Field | Value |
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Namespace | Cellular component |
Short description | Nuclear cap binding complex |
Full defintion | A conserved heterodimeric protein complex that binds to the 5' terminal cap structure m7G(5')ppp(5')N of nascent eukaryotic RNA polymerase II transcripts such as pre-mRNA and U snRNA. The consists of proteins known as CBP20 and CBP80, binds to cap structures in the nucleus, and is involved in pre-mRNA splicing, 3'-end formation, and RNA nuclear export. |
Subterm of |
The relationship of GO:0005846 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0005846, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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PMID | Specificity of recognition of mRNA 5' cap by human nuclear cap-binding complex. RNA. 2005 Sep; 11 (9): 1355–63.PMID: 16043498 The heterodimeric nuclear cap-binding complex (CBC) binds to the mono-methylated 5' cap of eukaryotic RNA polymerase II transcripts such as mRNA and U snRNA. The binding is important for nuclear maturation of mRNAs and possibly in the first round of translation and nonsense-mediated decay. It is also essential for nuclear export of U snRNAs in metazoans. We report characterization by fluorescence spectroscopy of the recognition of 5' capped RNA by human CBC. The association constants (K(as)) for 17 mono- and dinucleotide cap analogs as well as for the oligomer m7GpppA(m2') pU(m2')pA(m2') cover the range from 1.8 x 10(6) M(-1) to 2.3 x 10(8) M(-1). Higher affinity for CBC is observed for the dinucleotide compared with mononucleotide analogs, especially for those containing a purine nucleoside next to m7G. The mRNA tetramer associates with CBC as tightly as the dinucleotide analogs. Replacement of Tyr138 by alanine in the CBP20 subunit of CBC reduces the cap affinity except for the mononucleotide analogs, consistent with the crystallographic observation of the second base stacking on this residue. Our spectroscopic studies showed that contrary to the other known cap-binding proteins, the first two nucleotides of a capped-RNA are indispensable for its specific recognition by CBC. Differences in the cap binding of CBC compared with the eukaryotic translation initiation factor 4E (eIF4E) are analyzed and discussed regarding replacement of CBC by eIF4E. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: nuclear cap-binding protein subunit 1-like [Glycine max] gi|356570247|ref|XP_003553301.1| | 4 | ||
– | Nuclear cap-binding protein [Nicotiana tabacum] gi|224460055|gb|ACN43578.1| | 3 | ||
– | RNA-binding protein; TAIR: AT5G44200.1 CAP-binding protein 20; Swiss-Prot: sp|Q9XFD1|NCBP2_ARATH Nuclear cap-binding protein subunit 2; TrEMBL-Plants: tr|I3SEA9|I3SEA9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0446900 | 3 | ||
– | Cap-binding protein; TAIR: AT5G44200.1 CAP-binding protein 20; Swiss-Prot: sp|Q9XFD1|NCBP2_ARATH Nuclear cap-binding protein subunit 2; TrEMBL-Plants: tr|I3SEA9|I3SEA9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0446900 | 3 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Biological process | MRNA cis splicing, via spliceosome | 1 | 25.00 |