Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
Field | Value |
---|---|
Namespace | Biological process |
Short description | Double-strand break repair via nonhomologous end joining |
Full defintion | The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends. This term covers instances of separate pathways, called classical (or canonical) and alternative nonhomologous end joining (C-NHEJ and A-NHEJ). These in turn may further branch into sub-pathways, but evidence is still unclear. |
Subterm of |
The relationship of GO:0006303 with other GO terms.
Relationship type | GO terms |
---|---|
Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0006303, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
---|---|
GOC | rph |
PMID | DNA ligase III and DNA ligase IV carry out genetically distinct forms of end joining in human somatic cells. DNA Repair (Amst). 2014 Sep; 21 (): 97–110.PMID: 24837021 Ku-dependent C-NHEJ (classic non-homologous end joining) is the primary DNA EJing (end joining) repair pathway in mammals. Recently, an additional EJing repair pathway (A-NHEJ; alternative-NHEJ) has been described. Currently, the mechanism of A-NHEJ is obscure although a dependency on LIGIII (DNA ligase III) is often implicated. To test the requirement for LIGIII in A-NHEJ we constructed a LIGIII conditionally-null human cell line using gene targeting. Nuclear EJing activity appeared unaffected by a deficiency in LIGIII as, surprisingly, so were random gene targeting integration events. In contrast, LIGIII was required for mitochondrial function and this defined the gene's essential activity. Human Ku:LIGIII and Ku:LIGIV (DNA ligase IV) double knockout cell lines, however, demonstrated that LIGIII is required for the enhanced A-NHEJ activity that is observed in Ku-deficient cells. Most unexpectedly, however, the majority of EJing events remained LIGIV-dependent. In conclusion, although human LIGIII has an essential function in mitochondrial maintenance, it is dispensable for most types of nuclear DSB repair, except for the A-NHEJ events that are normally suppressed by Ku. Moreover, we describe that a robust Ku-independent, LIGIV-dependent repair pathway exists in human somatic cells. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: ATP-dependent DNA helicase 2 subunit KU80-like isoform X1 [Cicer arietinum] gi|502146520|ref|XP_004506489.1| | 9 | ||
– | PREDICTED: ATP-dependent DNA helicase 2 subunit KU70-like [Glycine max] gi|356576925|ref|XP_003556580.1| | 8 | ||
– | ATP-dependent DNA helicase 2 subunit KU80; TAIR: AT1G48050.1 Ku80 family protein; Swiss-Prot: sp|Q9FQ09|KU80_ARATH ATP-dependent DNA helicase 2 subunit KU80; TrEMBL-Plants: tr|G7JDE1|G7JDE1_MEDTR ATP-dependent DNA helicase 2 subunit KU80; Found in the gene: LotjaGi3g1v0433200 | 9 | ||
– | ATP-dependent DNA helicase 2 subunit KU80; TAIR: AT1G48050.1 Ku80 family protein; Swiss-Prot: sp|Q9FQ09|KU80_ARATH ATP-dependent DNA helicase 2 subunit KU80; TrEMBL-Plants: tr|G7JDE1|G7JDE1_MEDTR ATP-dependent DNA helicase 2 subunit KU80; Found in the gene: LotjaGi3g1v0433200 | 9 | ||
– | ATP-dependent DNA helicase 2 subunit Ku70; TAIR: AT1G16970.1 ATP-dependent DNA helicase 2 subunit Ku70-like protein; Swiss-Prot: sp|Q9FQ08|KU70_ARATH ATP-dependent DNA helicase 2 subunit KU70; TrEMBL-Plants: tr|K7N5J8|K7N5J8_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0365500 | 8 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Cellular component | Ku70:Ku80 complex | 1 | 20.00 |