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Field | Value |
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Namespace | Cellular component |
Short description | Delta DNA polymerase complex |
Full defintion | A multimeric DNA polymerase enzyme complex which differs in composition amongst species; in humans it is a heterotetramer of four subunits of approximately 125, 50, 68 and 12kDa, while in S. cerevisiae, it has three different subunits which form a heterotrimer, and the active enzyme is a dimer of this heterotrimer. Functions in DNA replication, mismatch repair and excision repair. |
Subterm of |
The relationship of GO:0043625 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0043625, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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GOC | jl |
ISBN | Oxford Dictionary of Biochemistry and Molecular Biology · Oxford University Press, USA, 1997 · 762 pages Arranged in A-to-Z order, the more than 17,000 entries provide basic information about fundamental, physiochemical laws, chemical compounds, constants, and formulae. The Dictionary also describes the essential features of some 2,000 enzymes and proteins, the reactions they catalyze and the functions they perform. These entries also include filenames to facilitate the location of entries in databases of sequences and definitions of 950 abbreviations and symbols. Designed for students, teachers, researchers and other professionals in any area of the biomedical sciences, the Dictionary has been fully updated anf revised to incorporate new information discovered since the original edition was published in 1997. |
PMID | Reconstitution and characterization of the human DNA polymerase delta four-subunit holoenzyme. Biochemistry. 2002 Nov 5; 41 (44): 13133–42.PMID: 12403614 Mammalian DNA polymerase delta was originally characterized as a tightly associated heterodimer consisting of the catalytic subunit, p125, and the p50 subunit. Recently, two additional subunits, the third (p68) and fourth subunits (p12), have been identified. The heterotetrameric human pol delta complex was reconstituted by overexpression of the four subunits in Sf9 cells, followed by purification to near-homogeneity using FPLC chromatography. The properties of the four-subunit enzyme were shown to be functionally indistinguishable from those of pol delta isolated from calf thymus. The physicochemical properties of both the reconstituted heterotetramer and the heterodimer of the p125 and p50 subunits were examined by gel filtration and glycerol gradient ultracentrifugation. These studies show quite clearly that the heterodimer and heterotetramer complexes do not behave in solution as dimeric structures. This issue is of significance because several studies of the yeast pol delta complexes have indicated that the third subunit is able to bring about the dimerization of the pol delta complex. The heterodimer is only weakly stimulated by PCNA, whereas the heterotetramer is strongly stimulated to a level with a specific activity comparable to that of the calf thymus enzyme. These results resolve earlier, conflicting reports on the response of the heterodimer to PCNA. Nevertheless, the heterodimer does have some ability to interact functionally with PCNA, consistent with evidence that the p125 subunit itself has an ability to interact with PCNA. The functional interaction of PCNA with the pol delta complex may likely involve multiple contacts. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | DNA polymerase delta subunit [Medicago truncatula] gi|357452073|ref|XP_003596313.1| | 3 | ||
– | DNA polymerase delta subunit 3; TAIR: AT1G78650.1 DNA-directed DNA polymerase; Swiss-Prot: sp|Q9EQ28|DPOD3_MOUSE DNA polymerase delta subunit 3; TrEMBL-Plants: tr|I1LV82|I1LV82_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0274400 | 3 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Cellular component | Delta DNA polymerase complex | 1 | 50.00 |