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GO:0106073

View this GO term in other databases

Overview

Field Value
Namespace Molecular function
Short description Dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity
Full defintion Catalysis of the addition of the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation; the transfer of glucose from dolichyl phosphate glucose (Dol-P-Glc) on to the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol.
Subterm of

Relationships

The relationship of GO:0106073 with other GO terms.

Relationship type GO terms
Is a
Regulates n.a.
Part of n.a.
Positively regulates n.a.
Negatively regulates n.a.

Ancestor tree

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Additional data

This table contains additional metadata associated with the GO entry's definition field.

Field Value
GOCha
PMID
The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation.Burda P, Aebi M
Glycobiology. 1998 May; 8 (5): 455–62.PMID: 9597543

The biosynthesis of the lipid-linked oligosaccharide substrate for N-linked protein glycosylation follows a highly conserved pathway at the membrane of the endoplasmic reticulum. Based on the synthetic growth defect in combination with a reduced oligosaccharyltransferase activity (wbp1), we have identified alg10 mutant strains which accumulate lipid-linked Glc2Man9GlcNAc2. We cloned the corresponding wild-type gene and show in a novel in vitro assay that Alg10p is a dolichyl-phosphoglucose-dependent glucosyltransferase which adds the terminal alpha-1,2 glucose to the lipid-linked Glc2Man9GlcNAc2 oligosaccharide. Hypoglycosylation of secreted proteins in alg10 deletion strains demonstrates that the terminal alpha-1,2-linked glucose residue is a key element in substrate recognition by the oligosaccharyltransferase. This ensures that primarily completely assembled oligosaccharide is transferred to protein.

Associated Lotus transcripts 4

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

Transcript Name Description GO terms GO count
Lj0g3v0182329.1
– Alpha-1,2-glucosyltransferase ALG10-A [Medicago truncatula] gi|357505549|ref|XP_003623063.1|
GO terms
2
Lj0g3v0182329.2
– Alpha-1,2-glucosyltransferase ALG10-A [Medicago truncatula] gi|357505549|ref|XP_003623063.1|
GO terms
2
LotjaGi3g1v0224200.1
– Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; TAIR: AT5G02410.1 DIE2/ALG10 family; Swiss-Prot: sp|Q8L638|ALG10_ARATH Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; TrEMBL-Plants: tr|G7L3H5|G7L3H5_MEDTR Alpha-1,2-glucosyltransferase; Found in the gene: LotjaGi3g1v0224200
GO terms
2
LotjaGi3g1v0224200.2
– Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; TAIR: AT5G02410.1 DIE2/ALG10 family; Swiss-Prot: sp|Q8L638|ALG10_ARATH Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; TrEMBL-Plants: tr|G7L3H5|G7L3H5_MEDTR Alpha-1,2-glucosyltransferase; Found in the gene: LotjaGi3g1v0224200
GO terms
2

Co-occuring GO terms 1

A list of co-occurring GO terms within the L. japonicus gene space:

GO term Namespace Name Observations Saturation (%)
GO:0106073
Molecular function Dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity 1 25.00