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IPR004136 is a Nitronate monooxygenase.
<p>Nitronate monooxygenase (NMO), formerly referred to as 2-nitropropane dioxygenase (NPD) ([ec:1.13.11.32]), is an FMN-dependent enzyme that uses molecular oxygen to oxidise (anionic) alkyl nitronates and, in the case of the enzyme from Neurospora crassa, (neutral) nitroalkanes to the corresponding carbonyl compounds and nitrite. Previously classified as 2-nitropropane dioxygenase [[cite:PUB00019937], [cite:PUB00016932], [cite:PUB00041080]], but it is now recognised that this was the result of the slow ionisation of nitroalkanes to their nitronate (anionic) forms [[cite:PUB00075572]]. The enzymes from the fungus Neurospora crassa and the yeast Williopsis saturnus var. mrakii (formerly classified as Hansenula mrakii) contain non-covalently bound FMN as the cofactor. Active towards linear alkyl nitronates of lengths between 2 and 6 carbon atoms and, with lower activity, towards propyl-2-nitronate. The enzyme from N. crassa can also utilise neutral nitroalkanes, but with lower activity. One atom of oxygen is incorporated into the carbonyl group of the aldehyde product. The reaction appears to involve the formation of an enzyme-bound nitronate radical and an a-peroxynitroethane species, which then decomposes, either in the active site of the enzyme or after release, to acetaldehyde and nitrite.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Nitronate monooxygenase activity | Catalysis of the reaction: ethylnitronate + O(2) = acetaldehyde + nitrite. | ||
| Biological process | Oxidation-reduction process | A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: probable nitronate monooxygenase-like [Fragaria vesca subsp]. vesca] gi|470126409|ref|XP_004299180.1| | 6 | ||
| – | Aldolase-type TIM barrel family protein isoform 1 [Theobroma cacao] gi|508706851|gb|EOX98747.1| | 6 | ||
| – | 2-nitropropane dioxygenase-like protein [Medicago truncatula] gi|357460979|ref|XP_003600771.1| | 8 | ||
| – | Inosine-5'-monophosphate dehydrogenase; TAIR: AT5G64250.2 Aldolase-type TIM barrel family protein; Swiss-Prot: sp|O05413|2NPD_BACSU Probable nitronate monooxygenase; TrEMBL-Plants: tr|G7J9Z8|G7J9Z8_MEDTR 2-nitropropane dioxygenase-like protein; Found in the gene: LotjaGi4g1v0035700 | 8 | ||
| – | 2-nitropropane dioxygenase-like protein; TAIR: AT5G64250.2 Aldolase-type TIM barrel family protein; Swiss-Prot: sp|O05413|2NPD_BACSU Probable nitronate monooxygenase; TrEMBL-Plants: tr|K7L7D2|K7L7D2_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0351700 | 8 | ||
| – | Inosine-5'-monophosphate dehydrogenase; TAIR: AT5G64250.1 Aldolase-type TIM barrel family protein; Swiss-Prot: sp|Q9I4V0|2NPD_PSEAE Nitronate monooxygenase; TrEMBL-Plants: tr|K7L7D2|K7L7D2_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0351700 | 8 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd04730 | CDD | 1 | 16.67 |