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IPR006336 is a Glutamate--cysteine ligase, GCS2.
<p>Also known as gamma-glutamylcysteine synthetase and gamma-ECS ([ec:6.3.2.2]). This enzyme catalyses the first and rate limiting step in de novo glutathione biosynthesis. Members of this family are found in archaea, bacteria and plants. May and Leaver [[cite:PUB00014837]] discuss the possible evolutionary origins of glutamate-cysteine ligase enzymes in different organisms and suggest that it evolved independently in different eukaryotes, from an ancestral bacterial enzyme. They also state that Arabidopsis thaliana (Mouse-ear cress) gamma-glutamylcysteine synthetase is structurally unrelated to mammalian, yeast and Escherichia coli homologues. In plants, there are separate cytosolic and chloroplast forms of the enzyme.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Glutamate-cysteine ligase activity | Catalysis of the reaction: L-cysteine + L-glutamate + ATP = L-gamma-glutamyl-L-cysteine + ADP + 2 H(+) + phosphate. | ||
| Biological process | Cellular modified amino acid biosynthetic process | The chemical reactions and pathways resulting in the formation of compounds derived from amino acids, organic acids containing one or more amino substituents. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Gamma-glutamylcysteine synthetase [Lotus japonicus] gi|34484367|gb|AAO45821.1| | 8 | ||
| – | 8 | |||
| – | Glutamate-cysteine ligase; TAIR: AT4G23100.1 glutamate-cysteine ligase; Swiss-Prot: sp|P46309|GSH1_ARATH Glutamate--cysteine ligase, chloroplastic; TrEMBL-Plants: tr|G7KC28|G7KC28_MEDTR Glutamate-cysteine ligase B; Found in the gene: LotjaGi2g1v0434100 | 11 | ||
| – | Glutamate--cysteine ligase, chloroplastic; TAIR: AT4G23100.1 glutamate-cysteine ligase; Swiss-Prot: sp|Q9ZNX6|GSH1_MEDTR Glutamate--cysteine ligase, chloroplastic; TrEMBL-Plants: tr|Q6XXZ2|Q6XXZ2_LOTJA Gamma-glutamylcysteine synthetase; Found in the gene: LotjaGi4g1v0425600 | 11 | ||
| – | Glutamate--cysteine ligase, chloroplastic; TAIR: AT4G23100.1 glutamate-cysteine ligase; Swiss-Prot: sp|Q9ZNX6|GSH1_MEDTR Glutamate--cysteine ligase, chloroplastic; TrEMBL-Plants: tr|Q6XXZ2|Q6XXZ2_LOTJA Gamma-glutamylcysteine synthetase; Found in the gene: LotjaGi4g1v0425600 | 11 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| TIGR01436 | TIGRFAM | 1 | 20.00 |