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IPR019807 is a Hexokinase, binding site.
<p>Hexokinase ([ec:2.7.1.1]) [[cite:PUB00000566], [cite:PUB00001951]] is an important glycolytic enzyme that catalyses the phosphorylation of keto- and aldohexoses (e.g. glucose, mannose and fructose) using MgATP as the phosphoryl donor.</p> <p>In vertebrates there are four major isoenzymes, commonly referred as types I, II, III and IV. Type IV hexokinase, which is often incorrectly designated glucokinase [[cite:PUB00005375]], is only expressed in liver and pancreatic beta-cells and plays an important role in modulating insulin secretion; it is a protein of a molecular mass of about 50 Kd. Hexokinases of types I to III, which have low Km values for glucose, have a molecular mass of about 100 Kd. Structurally they consist of a very small N-terminal hydrophobic membrane-binding domain followed by two highly similar domains of 450 residues. The first domain has lost its catalytic activity and has evolved into a regulatory domain.</p> <p>In yeast there are three different isoenzymes: hexokinase PI (gene HXK1), PII (gene HXKB), and glucokinase (gene GLK1). All three proteins have a molecular mass of about 50 Kd.</p> <p>The hexokinase domain has an α/β fold and is distinctly folded in two subdomains of unequal size: the large and small subdomains. The large subdomain comprises a six-stranded mixed β-sheet and a number of additional α-helices. On one side, the sheet packs against the small subdomain, and on the other side it is shielded by several α-helices. The dominant feature of the small subdomain is a five stranded mixed β-sheet. The sheet is flanked by two helices on one side and by one helix on the other. The subdomain also has an additional β-sheet formed by two antiparallel strands [[cite:PUB00014215], [cite:PUB00036888]].</p> <p>All these enzymes contain one (or two in the case of types I to III isozymes) strongly conserved region which has been shown to be involved in substrate binding [[cite:PUB00000147]]. This entry represents the conserved region involved in substrate binding.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Hexokinase activity | Catalysis of the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate. | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Biological process | Glycolytic process | The chemical reactions and pathways resulting in the breakdown of a carbohydrate into pyruvate, with the concomitant production of a small amount of ATP and the reduction of NAD(P) to NAD(P)H. Glycolysis begins with the metabolism of a carbohydrate to generate products that can enter the pathway and ends with the production of pyruvate. Pyruvate may be converted to acetyl-coenzyme A, ethanol, lactate, or other small molecules. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: hexokinase-3-like [Glycine max] gi|356497339|ref|XP_003517518.1| | 23 | ||
| – | PREDICTED: hexokinase-1-like [Cicer arietinum] gi|502156108|ref|XP_004510315.1| | 20 | ||
| – | Phosphotransferase; TAIR: AT1G50460.1 hexokinase-like 1; Swiss-Prot: sp|Q9LPS1|HXK3_ARATH Hexokinase-3; TrEMBL-Plants: tr|I1JAF6|I1JAF6_SOYBN Phosphotransferase; Found in the gene: LotjaGi2g1v0443600 | 24 | ||
| – | Phosphotransferase; TAIR: AT3G20040.1 Hexokinase; Swiss-Prot: sp|Q56XE8|HXK4_ARATH Hexokinase-4; TrEMBL-Plants: tr|I1JAF6|I1JAF6_SOYBN Phosphotransferase; Found in the gene: LotjaGi2g1v0443600 | 24 | ||
| – | Phosphotransferase; TAIR: AT3G20040.1 Hexokinase; Swiss-Prot: sp|Q56XE8|HXK4_ARATH Hexokinase-4; TrEMBL-Plants: tr|I1JAF6|I1JAF6_SOYBN Phosphotransferase; Found in the gene: LotjaGi2g1v0443600 | 24 | ||
| – | Phosphotransferase; TAIR: AT4G29130.1 hexokinase 1; Swiss-Prot: sp|Q9SEK2|HXK1_TOBAC Hexokinase-1; TrEMBL-Plants: tr|V7ASM0|V7ASM0_PHAVU Phosphotransferase; Found in the gene: LotjaGi3g1v0371800 | 23 | ||
| – | Phosphotransferase; TAIR: AT1G47840.1 hexokinase 3; Swiss-Prot: sp|Q6Q8A5|HXK2_TOBAC Hexokinase-2, chloroplastic; TrEMBL-Plants: tr|I1MY29|I1MY29_SOYBN Phosphotransferase; Found in the gene: LotjaGi5g1v0006300 | 21 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd00012 | CDD | 1 | 14.29 |