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GO:0000818

Overview

Field Value
Namespace Cellular component
Short description Nuclear MIS12/MIND complex
Full defintion A multiprotein kinetochore subcomplex that binds to centromeric chromatin and forms part of the inner kinetochore of a chromosome in the nucleus. It helps to recruit outer kinetochore subunits that will bind to microtubules. Nuclear localization arises in some organisms because the nuclear envelope is not broken down during mitosis. In S. cerevisiae, it consists of at least four proteins: Mtw1p, Nnf1p, Nsl1p, and Dsn1.
Subterm of

Relationships

The relationship of GO:0000818 with other GO terms.

Relationship type GO terms
Is a
Regulates n.a.
Part of
Positively regulates n.a.
Negatively regulates n.a.

Ancestor tree

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Additional data

This table contains additional metadata associated with the GO entry's definition field.

Field Value
GOCse
PMID
Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes.
Genes Dev. ; 17 (23): 2902–21.PMID: 14633972

Kinetochores are multiprotein complexes that assemble on centromeric DNA and attach chromosomes to spindle microtubules. Over the past six years, the number of proteins known to localize to the Saccharomyces cerevisiae kinetochore has increased from around 10 to over 60. However, relatively little is known about the protein-protein interactions that mediate kinetochore assembly or about the overall structure of microtubule-attachment sites. Here we used biophysical techniques, affinity purification, mass spectrometry, and in vivo assays to examine the state of association of 31 centromere-binding proteins, including six proteins newly identified as kinetochore subunits. We found that yeast kinetochores resemble transcriptional enhancers in being composed of at least 17 discrete subcomplexes that assemble on DNA to form a very large structure with a mass in excess of 5 MD. Critical to kinetochore assembly are proteins that bridge subunits in direct contact with DNA and subunits bound to microtubules. We show that two newly identified kinetochore complexes, COMA (Ctf19p-Okp1p-Mcm21p-Ame1p) and MIND (Mtw1p including Nnf1p-Nsl1p-Dsn1p) function as bridges. COMA, MIND, and the previously described Ndc80 complex constitute three independent and essential platforms onto which outer kinetochore proteins assemble. In addition, we propose that the three complexes have different functions with respect to force generation and MT attachment.

Associated Lotus transcripts 2

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

Transcript Name Description GO terms GO count
Protein embryo defective 3006 [Arabidopsis thaliana] gi|18415295|ref|NP_567582.1| 1
Embryo defective 3006 protein, putative; TAIR: AT4G19350.1 embryo defective 3006; Swiss-Prot: sp|Q39HN2|RLME_BURL3 Ribosomal RNA large subunit methyltransferase E; TrEMBL-Plants: tr|K7MGX6|K7MGX6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0131800 1

Co-occuring GO terms 1

A list of co-occurring GO terms within the L. japonicus gene space:

GO term Namespace Name Observations Saturation (%)
Cellular component Nuclear MIS12/MIND complex 1 50.00