Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
Field | Value |
---|---|
Namespace | Cellular component |
Short description | MRNA cleavage and polyadenylation specificity factor complex |
Full defintion | A multisubunit complex that binds to the canonical AAUAAA hexamer and to U-rich upstream sequence elements on the pre-mRNA, thereby stimulating the otherwise weakly active and nonspecific polymerase to elongate efficiently RNAs containing a poly(A) signal. |
Subterm of |
The relationship of GO:0005847 with other GO terms.
Relationship type | GO terms |
---|---|
Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0005847, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
---|---|
PMID | Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. EMBO J. 2004 Feb 11; 23 (3): 616–26.PMID: 14749727 In mammals, polyadenylation of mRNA precursors (pre-mRNAs) by poly(A) polymerase (PAP) depends on cleavage and polyadenylation specificity factor (CPSF). CPSF is a multisubunit complex that binds to the canonical AAUAAA hexamer and to U-rich upstream sequence elements on the pre-mRNA, thereby stimulating the otherwise weakly active and nonspecific polymerase to elongate efficiently RNAs containing a poly(A) signal. Based on sequence similarity to the Saccharomyces cerevisiae polyadenylation factor Fip1p, we have identified human Fip1 (hFip1) and found that the protein is an integral subunit of CPSF. hFip1 interacts with PAP and has an arginine-rich RNA-binding motif that preferentially binds to U-rich sequence elements on the pre-mRNA. Recombinant hFip1 is sufficient to stimulate the in vitro polyadenylation activity of PAP in a U-rich element-dependent manner. hFip1, CPSF160 and PAP form a ternary complex in vitro, suggesting that hFip1 and CPSF160 act together in poly(A) site recognition and in cooperative recruitment of PAP to the RNA. These results show that hFip1 significantly contributes to CPSF-mediated stimulation of PAP activity. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: cleavage and polyadenylation specificity factor subunit 2-like isoform 2 [Glycine max] gi|356530858|ref|XP_003533996.1| | 3 | ||
– | PREDICTED: cleavage and polyadenylation specificity factor subunit 2-like isoform 1 [Glycine max] gi|356559788|ref|XP_003548179.1| | 3 | ||
– | Cleavage and polyadenylation specificity factor subunit 2; TAIR: AT5G23880.1 cleavage and polyadenylation specificity factor 100; Swiss-Prot: sp|Q9LKF9|CPSF2_ARATH Cleavage and polyadenylation specificity factor subunit 2; TrEMBL-Plants: tr|A0A1J7HZX4|A0A1J7HZX4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0141300 | 3 | ||
– | Cleavage and polyadenylation specificity factor subunit 2; TAIR: AT5G23880.1 cleavage and polyadenylation specificity factor 100; Swiss-Prot: sp|Q9LKF9|CPSF2_ARATH Cleavage and polyadenylation specificity factor subunit 2; TrEMBL-Plants: tr|A0A1J7HZX4|A0A1J7HZX4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0141300 | 3 | ||
– | Cleavage and polyadenylation specificity factor subunit 2-like protein; TAIR: AT5G23880.1 cleavage and polyadenylation specificity factor 100; Swiss-Prot: sp|Q9LKF9|CPSF2_ARATH Cleavage and polyadenylation specificity factor subunit 2; TrEMBL-Plants: tr|A0A0B0PTU3|A0A0B0PTU3_GOSAR Cleavage and polyadenylation specificity factor subunit 2-like protein; Found in the gene: LotjaGi2g1v0141300 | 3 | ||
– | Cleavage and polyadenylation specificity factor subunit 2; TAIR: AT5G23880.1 cleavage and polyadenylation specificity factor 100; Swiss-Prot: sp|Q9LKF9|CPSF2_ARATH Cleavage and polyadenylation specificity factor subunit 2; TrEMBL-Plants: tr|A0A1J7HZX4|A0A1J7HZX4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0141300 | 3 | ||
– | Cleavage and polyadenylation specificity factor subunit 2; TAIR: AT5G23880.1 cleavage and polyadenylation specificity factor 100; Swiss-Prot: sp|Q9LKF9|CPSF2_ARATH Cleavage and polyadenylation specificity factor subunit 2; TrEMBL-Plants: tr|A0A1J7HZX4|A0A1J7HZX4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0141300 | 3 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Biological process | MRNA cleavage | 1 | 14.29 |