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Field | Value |
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Namespace | Cellular component |
Short description | RNA polymerase I core factor complex |
Full defintion | A RNA polymerase I-specific transcription factor complex that is required for the transcription of rDNA by RNA polymerase I. In yeast the complex consists of Rrn6p, Rrn7p, and Rrn11p. |
Subterm of |
The relationship of GO:0070860 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0070860, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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PMID | RRN11 encodes the third subunit of the complex containing Rrn6p and Rrn7p that is essential for the initiation of rDNA transcription by yeast RNA polymerase I. J Biol Chem. 1996 Aug 30; 271 (35): 21062–7.PMID: 8702872 A new gene, RRN11, has been defined by certain rrn mutants of Saccharomyces cerevisiae which are defective specifically in the transcription of 35 S rRNA gene by RNA polymerase I (pol I). We have cloned the gene and found that it encodes a protein of 507 amino acids. We have used a strain with the chromosomal RRN11 deleted and carrying HA1 epitope-tagged RRN11 on a plasmid to isolate a protein complex containing the protein encoded by RRN11. This protein complex complemented rrn6 mutant extracts, which were previously shown to be deficient in the essential pol I transcription factor called Rrn6/7 complex or core factor (CF). The CF complex was previously shown to consist of three proteins, the 102- and 60-kDa subunits encoded by RRN6 and RRN7, respectively, and the 66-kDa subunit. The results of the above complementation experiments combined with mobility of Rrn11p in SDS-polyacrylamide gel electrophoresis analysis relative to Rrn6p and Rrn7p led to the conclusion that RRN11 encodes the 66-kDa subunit of CF. Glutathione S-transferase-Rrn11p fusion protein was found to bind strongly to 35S-labeled Rrn6p and Rrn7p but only weakly to 35S-labeled TATA-binding protein. Similarly, glutathione S-transferase-Rrn7p fusion protein bound strongly to 35S-labeled Rrn6p and Rrn11p but only weakly to 35S-labeled TATA-binding protein. These results are consistent with the fact that one can purify CF consisting of Rrn6p, Rrn7p, and Rrn11p from yeast cell extracts, but the purified complex does not contain TATA-binding protein. RRN11 was shown to be an essential gene, and [3H]uridine pulse experiments demonstrated directly that RRN11 is essential for rDNA transcription by pol I in vivo. Thus all three subunits of CF are essential for rDNA transcription. Because of the resemblance of CF to mammalian essential pol I transcription factor SL1, the amino acid sequences of Rrn11p and the other two subunits of CF were compared with those of the three TATA-binding protein-associated factors (TAFs) in the human SL1, TAFI48, TAFI63, and TAFI110. No significant similarity was detected between two sets of the proteins. Similarity as well as differences between CF and SL1 are discussed. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: TATA box-binding protein-associated factor RNA polymerase I subunit B-like [Cicer arietinum] gi|502149384|ref|XP_004507506.1| | 4 | ||
– | PREDICTED: TATA box-binding protein-associated factor RNA polymerase I subunit B-like [Cicer arietinum] gi|502149384|ref|XP_004507506.1| | 4 | ||
– | TATA box-binding protein associated factor RNA polymerase I subunit B-like protein; TAIR: AT2G02955.1 maternal effect embryo arrest 12; Swiss-Prot: sp|Q5XVF0|MEE12_ARATH TATA box-binding protein-associated factor RNA polymerase I subunit B; TrEMBL-Plants: tr|I1LJ67|I1LJ67_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0514500 | 4 | ||
– | TATA box-binding protein associated factor RNA polymerase I subunit B-like protein; TAIR: AT2G02955.1 maternal effect embryo arrest 12; Swiss-Prot: sp|Q5XVF0|MEE12_ARATH TATA box-binding protein-associated factor RNA polymerase I subunit B; TrEMBL-Plants: tr|I1LJ67|I1LJ67_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0514500 | 4 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Cellular component | RNA polymerase I core factor complex | 1 | 25.00 |