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IPR006746 is a 26S proteasome non-ATPase regulatory subunit Rpn12.
<p>Intracellular proteins, including short-lived proteins such as cyclin, Mos, Myc, p53, NF-kappaB, and IkappaB, are degraded by the ubiquitin-proteasome system. The 26S proteasome is a self-compartmentalising protease responsible for the regulated degradation of intracellular proteins in eukaryotes [[cite:PUB00016866], [cite:PUB00034667]]. This giant intracellular protease is formed by several subunits arranged into two 19S polar caps, where protein recognition and ATP-dependent unfolding occur, flanking a 20S central barrel-shaped structure with an inner proteolytic chamber. This overall structure is highly conserved among eukaryotes and is essential for cell viability. Proteins targeted to the 26S proteasome are conjugated with a polyubiquitin chain by an enzymatic cascade before delivery to the 26S proteasome for degradation into oligopeptides.</p> <p>The 26S proteasome can be divided into two subcomplexes: the 19S regulatory particle (RP) and the 20S core particle (CP) [[cite:PUB00086026]]. The 19S component is divided into a "base" subunit containing six ATPases (Rpt proteins) and two non-ATPases (Rpn1, Rpn2), and a "lid" subunit composed of eight stoichiometric proteins (Rpn3, Rpn5, Rpn6, Rpn7, Rpn8, Rpn9, Rpn11, Rpn12) [[cite:PUB00034668]]. Additional non-essential and species specific proteins may also be present. The 19S unit performs several essential functions including binding the specific protein substrates, unfolding them, cleaving the attached ubiquitin chains, opening the 20S subunit, and driving the unfolded polypeptide into the proteolytic chamber for degradation. The 26s proteasome and 19S regulator are of medical interest due to their involvement in burn rehabilitation [[cite:PUB00043308]].</p> <p>This entry represents Rpn12 (also often annotated as 26S proteasome non-ATPase regulatory subunit 8). This protein has been shown to be important for the transition from metaphase to anaphase and the activation of Cdc28p kinase in yeast [[cite:PUB00020294], [cite:PUB00034669]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Cellular component | Proteasome regulatory particle | A multisubunit complex, which caps one or both ends of the proteasome core complex. This complex recognizes and unfolds ubiquitinated proteins, and translocates them to the proteasome core complex. | ||
| Biological process | Proteolysis | The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: 26S proteasome non-ATPase regulatory subunit RPN12A-like [Glycine max] gi|356524925|ref|XP_003531078.1| | 4 | ||
| – | PREDICTED: 26S proteasome non-ATPase regulatory subunit RPN12A [Vitis vinifera] gi|225426245|ref|XP_002264255.1| | 4 | ||
| – | PREDICTED: 26S proteasome non-ATPase regulatory subunit RPN12A-like [Glycine max] gi|356524925|ref|XP_003531078.1| | 4 | ||
| – | 26S proteasome non-ATPase regulatory subunit family protein; TAIR: AT1G64520.1 regulatory particle non-ATPase 12A; Swiss-Prot: sp|Q9SGW3|PSD8A_ARATH 26S proteasome non-ATPase regulatory subunit 8 homolog A; TrEMBL-Plants: tr|A0A0S3R0N9|A0A0S3R0N9_PHAAN Uncharacterized protein; Found in the gene: LotjaGi3g1v0466400 | 7 | ||
| – | 26S proteasome non-ATPase regulatory subunit family protein; TAIR: AT1G64520.1 regulatory particle non-ATPase 12A; Swiss-Prot: sp|Q9SGW3|PSD8A_ARATH 26S proteasome non-ATPase regulatory subunit 8 homolog A; TrEMBL-Plants: tr|A0A0S3R0N9|A0A0S3R0N9_PHAAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0025800 | 7 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| PTHR12387 | PANTHER | 1 | 20.00 |