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IPR007872 is a DPH-type metal-binding domain.
<p>This entry represents the DPH-type metal binding domain consists of a three-stranded β-sandwich with one sheet comprising two parallel strands: (i) β1 and (ii) β6 and one antiparallel strand: β5. The second sheet in the β-sandwich is comprised of strands β2, β3, and β4 running anti-parallel to each other. The two β-sheets are separated by a short stretch α-helix. It can be found in proteins such as DPH3 and DPH4. This domain is also found associated with N-terminal domain of heat shock protein DnaJ [interpro:IPR001623] domain [[cite:PUB00021079], [cite:PUB00093536], [cite:PUB00093772]].</p> <p>Diphthamide is a unique post-translationally modified histidine residue found only in translation elongation factor 2 (eEF-2). It is conserved from archaea to humans and serves as the target for diphteria toxin and Pseudomonas exotoxin A. These two toxins catalyse the transfer of ADP-ribose to diphtamide on eEF-2, thus inactivating eEF-2, halting cellular protein synthesis, and causing cell death [[cite:PUB00017080]]. The biosynthesis of diphtamide is dependent on at least five proteins, DPH1 to -5, and a still unidentified amidating enzyme. DPH3 and DPH4 share a conserved region, which encode a putative zinc finger, the DPH-type or CSL-type (after the the final conserved cysteine of the zinc finger and the next two residues) MB domain contains a Cys-X-Cys...Cys-X2-Cys motif which tetrahedrically coordinates both Fe and Zn. The Fe containing DPH-type MBD has an electron transfer activity [[cite:PUB00017069], [cite:PUB00017070], [cite:PUB00021079], [cite:PUB00046549], [cite:PUB00093536], [cite:PUB00093772]].</p>
This description is obtained from EB-eye REST.
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: DPH3 homolog [Cicer arietinum] gi|502149049|ref|XP_004507373.1| | 7 | ||
| – | Chaperone DnaJ; TAIR: AT4G10130.1 DNAJ heat shock N-terminal domain-containing protein; Swiss-Prot: sp|Q54CI5|DPH4_DICDI DPH4 homolog; TrEMBL-Plants: tr|V7AEJ7|V7AEJ7_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0186900 | 17 | ||
| – | Diphthamide biosynthesis protein 3; TAIR: AT2G15910.1 CSL zinc finger domain-containing protein; Swiss-Prot: sp|Q6CMG4|DPH3_KLULA Diphthamide biosynthesis protein 3; TrEMBL-Plants: tr|I3SPP6|I3SPP6_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0522200 | 9 | ||
| – | Diphthamide biosynthesis protein 3; TAIR: AT2G15910.1 CSL zinc finger domain-containing protein; Swiss-Prot: sp|Q6CMG4|DPH3_KLULA Diphthamide biosynthesis protein 3; TrEMBL-Plants: tr|I3SPP6|I3SPP6_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0502600 | 9 | ||
| – | Chaperone DnaJ; TAIR: AT4G10130.1 DNAJ heat shock N-terminal domain-containing protein; Swiss-Prot: sp|Q54CI5|DPH4_DICDI DPH4 homolog; TrEMBL-Plants: tr|A0A151TMW8|A0A151TMW8_CAJCA Diphthamide biosynthesis protein 4; Found in the gene: LotjaGi3g1v0526800 | 17 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd06257 | CDD | 1 | 20.00 |