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IPR016300 is a Arsenical pump ATPase, ArsA/GET3.
<p>Active extrusion is a common mechanism for the detoxification of heavy metals, drugs and antibiotics in bacteria, protozoa and mammals. This is particularly important for arsenic extrusion because of its prevalence in the environment and its potential to cause health and environmental problems. In prokaryotes, arsenic detoxification is accomplished by chromosomal and plasmid-borne operon-encoded efflux systems. ArsA from Escherichia coli is the catalytic subunit of the ArsAB extrusion pump, providing resistance to arsenite and antimonite. This pump consists of a soluble ATPase (ArsA) and a membrane channel (ArsB). Maintenance of a low intracellular concentration of oxidation produces resistance to the toxic agents. A third protein, ArsC, expands the substrate specificity to allow for arsenate resistance. ArsC reduces arsenate to arsenite, which is subsequently pumped out of the cell [[cite:PUB00007982]]. ArsA contains two nucleotide-binding sites (NBSs) and a binding site for arsenic or antimony. Binding of metalloids to the pump stimulates the ATPase activity [[cite:PUB00024783]].</p> <p>Homologues of the bacterial ArsA ATPase are found in eukaryotes, where they have several recognised functions unrelated to arsenic resistance [[cite:PUB00056214]]. Caenorhabditis elegans homologue Asna-1 is required for defence against arsenite and antimonite toxicity [[cite:PUB00043521]], and may be also involved in insulin signaling [[cite:PUB00056212]]. The homologue in yeast, GET3/Arr4, is part of the GET complex and not only is involved in stress tolerance to metals and heat [[cite:PUB00056211]], but also specifically recognises transmenbrane domains of tail-anchored (TA) proteins destined for the secretory pathway [[cite:PUB00056213]]. Archaeal GET3 homologues have also been discovered, suggesting that that archaea may possess a TA protein targeting pathway similar to that in eukaryotes [[cite:PUB00057862], [cite:PUB00057861]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Molecular function | ATPase activity | Catalysis of the reaction: ATP + H2O = ADP + phosphate + 2 H+. May or may not be coupled to another reaction. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: ATPase ASNA1 homolog [Cicer arietinum] gi|502157463|ref|XP_004510885.1| | 13 | ||
| – | PREDICTED: ATPase ASNA1 homolog [Cicer arietinum] gi|502157463|ref|XP_004510885.1| | 12 | ||
| – | P-loop containing nucleoside triphosphate hydrolases superfamily protein isoform 1 [Theobroma cacao] gi|508710257|gb|EOY02154.1| | 14 | ||
| – | P-loop containing nucleoside triphosphate hydrolases superfamily protein [Arabidopsis thaliana] gi|30681260|ref|NP_187646.2| | 9 | ||
| – | ATPase ASNA1-like protein [Medicago truncatula] gi|357443179|ref|XP_003591867.1| | 8 | ||
| – | ATPase ASNA1 homolog; TAIR: AT1G01910.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein; Swiss-Prot: sp|A7RQM5|ASNA_NEMVE ATPase ASNA1 homolog; TrEMBL-Plants: tr|I3SS77|I3SS77_LOTJA ATPase ASNA1 homolog; Found in the gene: LotjaGi3g1v0375100 | 13 | ||
| – | ATPase ASNA1; TAIR: AT3G10350.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein; Swiss-Prot: sp|O27555|ARSA_METTH Putative arsenical pump-driving ATPase; TrEMBL-Plants: tr|A0A072UVY6|A0A072UVY6_MEDTR Arsenical pump-driving ATPase-like protein; Found in the gene: LotjaGi3g1v0488500 | 10 | ||
| – | ATPase ASNA1; TAIR: AT3G10350.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein; Swiss-Prot: sp|O27555|ARSA_METTH Putative arsenical pump-driving ATPase; TrEMBL-Plants: tr|G7I505|G7I505_MEDTR Arsenical pump-driving ATPase-like protein; Found in the gene: LotjaGi5g1v0272400 | 12 | ||
| – | ATPase ASNA1; TAIR: AT3G10350.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein; Swiss-Prot: sp|O27555|ARSA_METTH Putative arsenical pump-driving ATPase; TrEMBL-Plants: tr|I1NHH3|I1NHH3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0272400 | 11 | ||
| – | ATPase ASNA1; TAIR: AT3G10350.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein; Swiss-Prot: sp|O27555|ARSA_METTH Putative arsenical pump-driving ATPase; TrEMBL-Plants: tr|A0A1J7FNR1|A0A1J7FNR1_LUPAN Uncharacterized protein; Found in the gene: LotjaGi5g1v0272400 | 11 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd02035 | CDD | 1 | 10.00 |