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IPR020548 is a Fructose-1,6-bisphosphatase, active site.
<p>This entry represents fructose-1,6-bisphosphatase (FBPase), a critical regulatory enzyme in gluconeogenesis that catalyses the removal of 1-phosphate from fructose 1,6-bis-phosphate to form fructose 6-phosphate [[cite:PUB00000153], [cite:PUB00000179]]. It is involved in many different metabolic pathways and found in most organisms. FBPase requires metal ions for catalysis (Mg<sup>2+</sup>and Mn<sup>2+</sup>being preferred) and the enzyme is potently inhibited by Li<sup>+</sup>. The fold of fructose-1,6-bisphosphatase was noted to be identical to that of inositol-1-phosphatase (IMPase) [[cite:PUB00000219]]. Inositol polyphosphate 1-phosphatase (IPPase), IMPase and FBPase share a sequence motif (Asp-Pro-Ile/Leu-Asp-Gly/Ser-Thr/Ser) which has been shown to bind metal ions and participate in catalysis. This motif is also found in the distantly-related fungal, bacterial and yeast IMPase homologues. It has been suggested that these proteins define an ancient structurally conserved family involved in diverse metabolic pathways, including inositol signalling, gluconeogenesis, sulphate assimilation and possibly quinone metabolism [[cite:PUB00004864]].</p> <p>In mammalian FBPase, a lysine residue has been shown to be involved in the catalytic mechanism [[cite:PUB00003256]]. The region around this residue is highly conserved and can be used as a signature pattern for FBPase and sedoheptulose-1,7-bisphosphatase (SBPase) an enzyme found plant chloroplasts and in photosynthetic bacteria that is functionally and structurally related to FBPase [[cite:PUB00001414]]. SBPase catalyses the hydrolysis of sedoheptulose 1,7-bisphosphate to sedoheptulose 7-phosphate, a step in the Calvin's reductive pentose phosphate cycle. This signature contains the active site lysine, however, it must be noted that, in some bacterial FBPase sequences, the active site lysine is replaced by an arginine.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | Carbohydrate metabolic process | The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule. | ||
| Molecular function | Phosphoric ester hydrolase activity | Catalysis of the reaction: RPO-R' + H2O = RPOOH + R'H. This reaction is the hydrolysis of any phosphoric ester bond, any ester formed from orthophosphoric acid, O=P(OH)3. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: sedoheptulose-1,7-bisphosphatase, chloroplastic-like isoform 2 [Glycine max] gi|356568654|ref|XP_003552525.1| | 7 | ||
| – | PREDICTED: fructose-1,6-bisphosphatase, cytosolic-like [Cicer arietinum] gi|502180459|ref|XP_004516643.1| | 16 | ||
| – | PREDICTED: fructose-1,6-bisphosphatase, chloroplastic-like [Glycine max] gi|356568028|ref|XP_003552216.1| | 21 | ||
| – | Fructose-1,6-bisphosphatase [Pisum sativum] gi|5305145|emb|CAB46084.1| | 17 | ||
| – | PREDICTED: fructose-1,6-bisphosphatase, cytosolic-like [Glycine max] gi|356525912|ref|XP_003531565.1| | 16 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT3G54050.1 high cyclic electron flow 1; Swiss-Prot: sp|P46275|F16P1_PEA Fructose-1,6-bisphosphatase, chloroplastic; TrEMBL-Plants: tr|I3T2L8|I3T2L8_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0574000 | 21 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT1G43670.1 Inositol monophosphatase family protein; Swiss-Prot: sp|P46276|F16P2_SOLTU Fructose-1,6-bisphosphatase, cytosolic; TrEMBL-Plants: tr|I3S7B9|I3S7B9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi2g1v0132900 | 16 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT1G43670.1 Inositol monophosphatase family protein; Swiss-Prot: sp|P46276|F16P2_SOLTU Fructose-1,6-bisphosphatase, cytosolic; TrEMBL-Plants: tr|I3S7B9|I3S7B9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi2g1v0132900 | 16 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT1G43670.1 Inositol monophosphatase family protein; Swiss-Prot: sp|P46276|F16P2_SOLTU Fructose-1,6-bisphosphatase, cytosolic; TrEMBL-Plants: tr|I3S7B9|I3S7B9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi2g1v0132900 | 17 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT1G43670.1 Inositol monophosphatase family protein; Swiss-Prot: sp|P46276|F16P2_SOLTU Fructose-1,6-bisphosphatase, cytosolic; TrEMBL-Plants: tr|I3S7B9|I3S7B9_LOTJA Uncharacterized protein; Found in the gene: LotjaGi2g1v0132900 | 16 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT1G43670.1 Inositol monophosphatase family protein; Swiss-Prot: sp|P46276|F16P2_SOLTU Fructose-1,6-bisphosphatase, cytosolic; TrEMBL-Plants: tr|A0A059BU66|A0A059BU66_EUCGR Uncharacterized protein; Found in the gene: LotjaGi5g1v0291200 | 16 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT1G43670.1 Inositol monophosphatase family protein; Swiss-Prot: sp|P46276|F16P2_SOLTU Fructose-1,6-bisphosphatase, cytosolic; TrEMBL-Plants: tr|A0A0B2QB13|A0A0B2QB13_GLYSO Fructose-1,6-bisphosphatase, cytosolic; Found in the gene: LotjaGi5g1v0291200 | 16 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT3G55800.1 sedoheptulose-bisphosphatase; Swiss-Prot: sp|O20252|S17P_SPIOL Sedoheptulose-1,7-bisphosphatase, chloroplastic; TrEMBL-Plants: tr|I1MZ53|I1MZ53_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0070200 | 14 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT3G55800.1 sedoheptulose-bisphosphatase; Swiss-Prot: sp|O20252|S17P_SPIOL Sedoheptulose-1,7-bisphosphatase, chloroplastic; TrEMBL-Plants: tr|I1MZ53|I1MZ53_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0070200 | 13 | ||
| – | Fructose-1,6-bisphosphatase class 1; TAIR: AT1G43670.1 Inositol monophosphatase family protein; Swiss-Prot: sp|A2WXB2|F16P2_ORYSI Fructose-1,6-bisphosphatase, cytosolic; TrEMBL-Plants: tr|I1KUG8|I1KUG8_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0356500 | 16 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd00354 | CDD | 1 | 6.67 |