Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR034556 is a S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase.
<p>This entry respresents S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase Tyw1 from eukaryotes and archaea. They are involved in the pathway wybutosine-tRNA(Phe) biosynthesis. It binds one [4Fe-4S] cluster and one [2Fe-2S] cluster. The [4Fe-4S] cluster is coordinated with three cysteines and an exchangeable S-adenosyl-L-methionine [[cite:PUB00050155], [cite:PUB00031322]]. The two carbon source required for this reaction has been identified as pyruvate. The exact role of the SAM molecule has yet to be determined. There appear to be two distinct sets of proteins with different multi-domain architectures, however they are sufficiently similar in the Radical SAM portion that they remain a single cluster [[cite:PUB00083703]].</p> <p>Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. It is important for translational reading-frame maintenance [[cite:PUB00044611]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | TRNA processing | The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group. | ||
| Molecular function | 4 iron, 4 sulfur cluster binding | Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | NADPH--cytochrome P450 reductase; TAIR: AT1G75200.1 flavodoxin family protein / radical SAM domain-containing protein; Swiss-Prot: sp|Q8RXN5|TYW1_ARATH S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; TrEMBL-Plants: tr|A0A0R0GAG6|A0A0R0GAG6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0016300 | 25 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 100.00 |