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IPR037381 is a E3 ubiquitin-protein ligase RFWD3.
<p>RFWD3 is a RING-type E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage [[cite:PUB00086834], [cite:PUB00086835], [cite:PUB00086836], [cite:PUB00153391], [cite:PUB00153396]].</p> <p>During ICL repair, single-stranded DNA (ssDNA) is generated. The trimeric ssDNA binding protein complex RPA coats the ssDNA. Subsequently, RAD51 is loaded onto RPA-bound ssDNA and catalyzes the critical activity in homologous recombination (HR). RFWD3 polyubiquitinates both RPA and RAD5, which increases their local turnover in DNA damage-induced foci to facilitate HR [[cite:PUB00086834]]. RFWD3-mediated ubiquitination of RPA has been shown to be essential for HR [[cite:PUB00086837]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Ubiquitin-protein transferase activity | Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages. | ||
| Cellular component | Nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. | ||
| Biological process | Protein ubiquitination | The process in which one or more ubiquitin groups are added to a protein. | ||
| Biological process | Interstrand cross-link repair | Removal of a DNA interstrand crosslink (a covalent attachment of DNA bases on opposite strands of the DNA) and restoration of the DNA. DNA interstrand crosslinks occur when both strands of duplex DNA are covalently tethered together (e.g. by an exogenous or endogenous agent), thus preventing the strand unwinding necessary for essential DNA functions such as transcription and replication. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | E3 ubiquitin-protein ligase RFWD3; TAIR: AT5G03450.1 Transducin/WD40 repeat-like superfamily protein; Swiss-Prot: sp|Q6PCD5|RFWD3_HUMAN E3 ubiquitin-protein ligase RFWD3; TrEMBL-Plants: tr|V7CZT3|V7CZT3_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0687400 | 6 | ||
| – | E3 ubiquitin-protein ligase RFWD3; TAIR: AT5G03450.1 Transducin/WD40 repeat-like superfamily protein; Swiss-Prot: sp|Q6PCD5|RFWD3_HUMAN E3 ubiquitin-protein ligase RFWD3; TrEMBL-Plants: tr|V7CZT3|V7CZT3_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0687400 | 6 | ||
| – | E3 ubiquitin-protein ligase RFWD3; TAIR: AT5G03450.1 Transducin/WD40 repeat-like superfamily protein; Swiss-Prot: sp|Q6PCD5|RFWD3_HUMAN E3 ubiquitin-protein ligase RFWD3; TrEMBL-Plants: tr|V7CZT3|V7CZT3_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0687400 | 7 | ||
| – | RING/U-box superfamily protein; TAIR: AT5G03450.1 Transducin/WD40 repeat-like superfamily protein; Swiss-Prot: sp|D2HWM5|RFWD3_AILME E3 ubiquitin-protein ligase RFWD3; TrEMBL-Plants: tr|V7CZT3|V7CZT3_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0687500 | 16 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 25.00 |