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Field | Value |
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Namespace | Cellular component |
Short description | AP-3 adaptor complex |
Full defintion | A heterotetrameric AP-type membrane coat adaptor complex that consists of beta3, delta, mu3 and sigma3 subunits and is found associated with endosomal membranes. AP-3 does not appear to associate with clathrin in all organisms. In at least humans, the AP-3 complex can be heterogeneric due to the existence of multiple subunit isoforms encoded by different genes (beta3A and beta3B, mu3A and mu3B, and sigma3A and sigma3B). |
Subterm of |
The relationship of GO:0030123 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0030123, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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GOC | mah |
PMID | Conservation and diversification of dileucine signal recognition by adaptor protein (AP) complex variants. J Biol Chem. 2011 Jan 21; 286 (3): 2022–30.PMID: 21097499 The clathrin-associated, heterotetrameric adaptor protein (AP) complexes, AP-1, AP-2, and AP-3, recognize signals in the cytosolic domains of transmembrane proteins, leading to their sorting to endosomes, lysosomes, lysosome-related organelles, and/or the basolateral membrane of polarized epithelial cells. One type of signal, referred to as "dileucine-based," fits the consensus motif (D/E)XXXL(L/I). Previous biochemical analyses showed that (D/E)XXXL(L/I) signals bind to a combination of two subunits of each AP complex, namely the AP-1 γ-σ1, AP-2 α-σ2, and AP-3 δ-σ3 hemicomplexes, and structural studies revealed that an imperfect variant of this motif lacking the (D/E) residue binds to a site straddling the interface of α and σ2. Herein, we report mutational and binding analyses showing that canonical (D/E)XXXL(L/I) signals bind to this same site on AP-2, and to similar sites on AP-1 and AP-3. The strength and amino acid requirements of different interactions depend on the specific signals and AP complexes involved. We also demonstrate the occurrence of diverse AP-1 heterotetramers by combinatorial assembly of various γ and σ1 subunit isoforms encoded by different genes. These AP-1 variants bind (D/E)XXXL(L/I) signals with marked preferences for certain sequences, implying that they are not functionally equivalent. Our results thus demonstrate that different AP complexes share a conserved binding site for (D/E)XXXL(L/I) signals. However, the characteristics of the binding site on each complex vary, providing for the specific recognition of a diverse repertoire of (D/E)XXXL(L/I) signals. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: AP-3 complex subunit sigma-like [Cicer arietinum] gi|502153217|ref|XP_004509260.1| | 2 | ||
– | PREDICTED: AP-3 complex subunit sigma-like [Cicer arietinum] gi|502153217|ref|XP_004509260.1| | 2 | ||
– | PREDICTED: AP-3 complex subunit sigma-like [Cicer arietinum] gi|502153217|ref|XP_004509260.1| | 2 | ||
– | PREDICTED: AP3-complex subunit beta-A-like [Glycine max] gi|356501063|ref|XP_003519348.1| | 2 | ||
– | PREDICTED: AP3-complex subunit beta-A-like [Glycine max] gi|356501063|ref|XP_003519348.1| | 2 | ||
– | PREDICTED: AP-3 complex subunit delta-like [Cicer arietinum] gi|502163538|ref|XP_004512869.1| | 2 | ||
– | Beta-adaptin-like protein; TAIR: AT3G55480.2 protein affected trafficking 2; Swiss-Prot: sp|Q9M2T1|AP3BA_ARATH AP3-complex subunit beta-A; TrEMBL-Plants: tr|K7KAL6|K7KAL6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0090500 | 2 | ||
– | AP-1 complex subunit sigma-like protein; TAIR: AT3G50860.1 Clathrin adaptor complex small chain family protein; Swiss-Prot: sp|Q8VZ37|AP3S_ARATH AP-3 complex subunit sigma; TrEMBL-Plants: tr|A0A072TSQ8|A0A072TSQ8_MEDTR AP-1 complex subunit sigma-like protein; Found in the gene: LotjaGi1g1v0416600 | 2 | ||
– | AP-3 complex subunit delta; TAIR: AT1G48760.1 delta-adaptin; Swiss-Prot: sp|Q9C744|AP3D_ARATH AP-3 complex subunit delta; TrEMBL-Plants: tr|G7L9R3|G7L9R3_MEDTR AP-3 complex subunit delta; Found in the gene: LotjaGi6g1v0173900 | 2 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Cellular component | AP-3 adaptor complex | 1 | 11.11 |