Gene Ontology — View

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GO:0043399

Overview

Field	Value
Namespace	Molecular function
Short description	TRNA A64-2'-O-ribosylphosphate transferase activity
Full defintion	Catalysis of the transfer of a phosphoribosyl group from 5'-phosphoribosyl-1'-pyrophosphate to position 64 of initiator tRNA.
Subterm of	GO:0016763 GO:0140101

Relationships

The relationship of GO:0043399 with other GO terms.

Relationship type	GO terms
Is a	GO:0016763 GO:0140101
Regulates	n.a.
Part of	n.a.
Positively regulates	n.a.
Negatively regulates	n.a.

Ancestor tree

A force layout showing the ancestor tree for GO:0043399, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.

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Additional data

This table contains additional metadata associated with the GO entry's definition field.

Field	Value
GOC	jl
PMID	Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination.Aström SU, Byström AS Cell. 1994 Nov 4; 79 (3): 535–46.PMID: 7954819 Using a genetic screen in yeast aimed at identifying cellular factors involved in initiator and elongator methionine tRNA discrimination in the translational process, we have identified a mutation that abolish the requirement for elongator methionine tRNA. The gene affected, which we call the ribosylation of the initiator tRNA gene or RIT1, encodes a 2'-O-ribosyl phosphate transferase. This enzyme modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. As the initiator tRNA participates both in the initiation and elongation of translation in a rit1 strain, we conclude that the 2'-O-ribosyl phosphate modification discriminates the initiator tRNAs from the elongator tRNAs during protein synthesis. The modification enzyme was shown to recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs.

Field

Value

GOC

PMID

Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination.Aström SU, Byström AS
Cell. 1994 Nov 4; 79 (3): 535–46.PMID: 7954819

Using a genetic screen in yeast aimed at identifying cellular factors involved in initiator and elongator methionine tRNA discrimination in the translational process, we have identified a mutation that abolish the requirement for elongator methionine tRNA. The gene affected, which we call the ribosylation of the initiator tRNA gene or RIT1, encodes a 2'-O-ribosyl phosphate transferase. This enzyme modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. As the initiator tRNA participates both in the initiation and elongation of translation in a rit1 strain, we conclude that the 2'-O-ribosyl phosphate modification discriminates the initiator tRNAs from the elongator tRNAs during protein synthesis. The modification enzyme was shown to recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs.

Associated Lotus transcripts 5

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on April 27, 2020, while the GO metadata was last updated on April 27, 2020.

Transcript	Name	Description	GO terms	GO count
Lj4g3v3044970.2 View transcript LORE1 insertions in MG20 v3.0 Genome browser Expression Atlas (ExpAt)	–	TRNA A64-2'-O-ribosylphosphate transferase [Medicago truncatula] gi\|357520857\|ref\|XP_003630717.1\|	GO terms View details of GO:0019988 View details of GO:0043399	2
LotjaGi4g1v0445500.1 View transcript LORE1 insertions in MG20 v3.0 Genome browser Expression Atlas (ExpAt)	–	Initiator tRNA phosphoribosyl-transferase, putative; TAIR: AT2G40570.1 initiator tRNA phosphoribosyl transferase family protein; Swiss-Prot: sp\|Q10181\|YAW6_SCHPO Uncharacterized protein C3F10.06c; TrEMBL-Plants: tr\|A0A1J7HBJ7\|A0A1J7HBJ7_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0445500	GO terms View details of GO:0019988 View details of GO:0043399	2
LotjaGi4g1v0445500.2 View transcript LORE1 insertions in MG20 v3.0 Genome browser Expression Atlas (ExpAt)	–	Initiator tRNA phosphoribosyl-transferase, putative; TAIR: AT2G40570.1 initiator tRNA phosphoribosyl transferase family protein; Swiss-Prot: sp\|Q10181\|YAW6_SCHPO Uncharacterized protein C3F10.06c; TrEMBL-Plants: tr\|A0A1J7HBJ7\|A0A1J7HBJ7_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0445500	GO terms View details of GO:0019988 View details of GO:0043399	2
LotjaGi4g1v0445500.3 View transcript LORE1 insertions in MG20 v3.0 Genome browser Expression Atlas (ExpAt)	–	Initiator tRNA phosphoribosyl-transferase, putative; TAIR: AT2G40570.1 initiator tRNA phosphoribosyl transferase family protein; Swiss-Prot: sp\|Q10181\|YAW6_SCHPO Uncharacterized protein C3F10.06c; TrEMBL-Plants: tr\|A0A1J7HBJ7\|A0A1J7HBJ7_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0445500	GO terms View details of GO:0019988 View details of GO:0043399	2
LotjaGi4g1v0445500.4 View transcript LORE1 insertions in MG20 v3.0 Genome browser Expression Atlas (ExpAt)	–	Initiator tRNA phosphoribosyl-transferase, putative; TAIR: AT2G40570.1 initiator tRNA phosphoribosyl transferase family protein; Swiss-Prot: sp\|Q10181\|YAW6_SCHPO Uncharacterized protein C3F10.06c; TrEMBL-Plants: tr\|A0A1J7HBJ7\|A0A1J7HBJ7_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0445500	GO terms View details of GO:0019988 View details of GO:0043399	2

Co-occuring GO terms 1

A list of co-occurring GO terms within the L. japonicus gene space:

GO term	Namespace	Name	Observations	Saturation (%)
GO:0043399 View details of GO:0043399 AmiGO InterPro QuickGO	Molecular function	TRNA A64-2'-O-ribosylphosphate transferase activity	1	20.00