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GO:0090730

View this GO term in other databases

Overview

Field Value
Namespace Cellular component
Short description Las1 complex
Full defintion A four subunit complex, that comprises all the necessary RNA processing enzymes (endonuclease, polynucleotide kinase, and exonuclease) to mediate 'cistronic rRNA transcript ITS2 (internal transcribed spacer) cleavage' (GO:0000448).
Subterm of

Relationships

The relationship of GO:0090730 with other GO terms.

Relationship type GO terms
Is a
Regulates n.a.
Part of n.a.
Positively regulates n.a.
Negatively regulates n.a.

Ancestor tree

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Additional data

This table contains additional metadata associated with the GO entry's definition field.

Field Value
GOCvw
PMID
Coordinated Ribosomal ITS2 RNA Processing by the Las1 Complex Integrating Endonuclease, Polynucleotide Kinase, and Exonuclease Activities.Gasse L, Flemming D, Hurt E
Mol Cell. 2015 Dec 3; 60 (5): 808–815.PMID: 26638174

The rapidly evolving internal transcribed spacer 2 (ITS2) in the pre-ribosomal RNA is one of the most commonly applied phylogenetic markers at species and genus level. Yet, during ribosome biogenesis ITS2 is removed in all eukaryotes by a common, but still unknown, mechanism. Here we describe the existence of an RNA processome, assembled from four conserved subunits, Las1-Grc3-Rat1-Rai1, that carries all the necessary RNA processing enzymes to mediate coordinated ITS2 rRNA removal. Las1 is the long-sought-after endonuclease cleaving 27SB pre-rRNA at site C2 to yield a 5'-OH end at the 26S pre-rRNA and 2',3' cyclic phosphate at the 3' end of 7S pre-rRNA. Subsequently, polynucleotide kinase Grc3 catalyzes ATP-dependent 5'-OH phosphorylation of 26S pre-rRNA, which in turn enables Rat1-Rai1 exonuclease to generate 25S' pre-rRNA. ITS2 processing is reminiscent of tRNA splicing, but instead of subsequent tRNA ligation, the Las1 complex carries along an exonuclease tool to degrade the ITS2 rRNA.

Associated Lotus transcripts 3

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

Transcript Name Description GO terms GO count
Lj2g3v3019670.1
– LAS1-like protein [Medicago truncatula] gi|357493865|ref|XP_003617221.1|
GO terms
3
Lj2g3v3019680.1
– LAS1-like protein [Medicago truncatula] gi|357493865|ref|XP_003617221.1|
GO terms
3
LotjaGi1g1v0052000.1
– Ribosomal biogenesis protein LAS1; TAIR: AT5G12220.1 las1-like family protein; Swiss-Prot: sp|Q9Y4W2|LAS1L_HUMAN Ribosomal biogenesis protein LAS1L; TrEMBL-Plants: tr|A0A151U1Y2|A0A151U1Y2_CAJCA LAS1-like protein; Found in the gene: LotjaGi1g1v0052000
GO terms
3

Co-occuring GO terms 1

A list of co-occurring GO terms within the L. japonicus gene space:

GO term Namespace Name Observations Saturation (%)
GO:0090730
Cellular component Las1 complex 1 33.33