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IPR002629 is a Cobalamin-independent methionine synthase MetE, C-terminal/archaeal.
<p>Methionine synthases catalyse the the final step of methionine biosynthesis. Two apparently unrelated families of proteins catalyse this step: cobalamin-dependent methionine synthase, which catalyses the transfer of a methyl group from N5-methyltetrahydrofolate to L-homocysteine and requires cobalamin as a cofactor (MetH; 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase; [ec:2.1.1.13]) and cobalamin-independent methionine synthase, which catalyses the transfer of a methyl group from methyltetrahydrofolate to L-homocysteine without using an intermediate methyl carrier (MetE; 5-methyltetrahydropteroyltri-L-glutamate:L-homocysteine S-methyltransferase; [ec:2.1.1.14]). These enzymes display no detectable sequence homology between them, but both require zinc for activation and binding to L-homocysteine. Organisms that cannot obtain cobalamin (vitamin B12) encode only the cobalamin-independent enzyme. Escherichia coli and many other bacteria express both enzymes [[cite:PUB00070291]]. Mammals utilise only cobalamin-dependent methionine synthase, while plants and yeasts utilise only the cobalamin-independent enzyme.</p> <p>The N-terminal half and C-terminal half of MetE in E. coli show some sequence similarity, indicating that the metE gene has evolved from an ancestral metE gene by duplication [[cite:PUB00070291]]. This entry represents a the C-terminal domain of cobalamin-independent methionine synthase (MetE) from bacteria and plants, which contains the zinc ion responsible for binding and activating homocysteine [[cite:PUB00037682]]. It also includes archaeal proteins where this domain corresponds to the entire length of the protein [[cite:PUB00070292]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity | Catalysis of the reaction: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamate. | ||
| Molecular function | Zinc ion binding | Interacting selectively and non-covalently with zinc (Zn) ions. | ||
| Biological process | Methionine biosynthetic process | The chemical reactions and pathways resulting in the formation of methionine (2-amino-4-(methylthio)butanoic acid), a sulfur-containing, essential amino acid found in peptide linkage in proteins. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Glycine max] gi|356547867|ref|XP_003542326.1| | 7 | ||
| – | PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Glycine max] gi|356514027|ref|XP_003525709.1| | 7 | ||
| – | PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like isoform 1 [Glycine max] gi|356571744|ref|XP_003554033.1| | 7 | ||
| – | PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like isoform 1 [Glycine max] gi|356571744|ref|XP_003554033.1| | 6 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|P93263|METE_MESCR 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|Q71EW8|Q71EW8_SOYBN Methionine synthase; Found in the gene: LotjaGi1g1v0646100 | 16 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|P93263|METE_MESCR 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|Q71EW8|Q71EW8_SOYBN Methionine synthase; Found in the gene: LotjaGi1g1v0646100 | 16 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|P93263|METE_MESCR 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|Q71EW8|Q71EW8_SOYBN Methionine synthase; Found in the gene: LotjaGi1g1v0646100 | 16 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|P93263|METE_MESCR 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|Q71EW8|Q71EW8_SOYBN Methionine synthase; Found in the gene: LotjaGi1g1v0646100 | 16 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|P93263|METE_MESCR 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|Q71EW8|Q71EW8_SOYBN Methionine synthase; Found in the gene: LotjaGi1g1v0646100 | 16 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|O50008|METE1_ARATH 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1; TrEMBL-Plants: tr|A0A151RFH6|A0A151RFH6_CAJCA 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Found in the gene: LotjaGi2g1v0109800 | 15 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|O50008|METE1_ARATH 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1; TrEMBL-Plants: tr|A0A151RFH6|A0A151RFH6_CAJCA 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Found in the gene: LotjaGi2g1v0109800 | 15 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|O50008|METE1_ARATH 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1; TrEMBL-Plants: tr|A0A151RFH6|A0A151RFH6_CAJCA 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Found in the gene: LotjaGi2g1v0109800 | 15 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|O50008|METE1_ARATH 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1; TrEMBL-Plants: tr|A0A151RFH6|A0A151RFH6_CAJCA 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Found in the gene: LotjaGi2g1v0109800 | 15 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|Q42699|METE_CATRO 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|I1LXY1|I1LXY1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0193300 | 20 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|Q42699|METE_CATRO 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|I1LXY1|I1LXY1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0193300 | 15 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|Q42699|METE_CATRO 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|I1LXY1|I1LXY1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0193300 | 15 | ||
| – | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TAIR: AT5G17920.1 Cobalamin-independent synthase family protein; Swiss-Prot: sp|Q42699|METE_CATRO 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; TrEMBL-Plants: tr|I1LXY1|I1LXY1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0193300 | 15 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd03312 | CDD | 1 | 5.88 |