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IPR012259

Source: InterPro

Description

IPR012259 is a Dihydrofolate reductase.

<p>Dihydrofolate reductase (DHFR) ([ec:1.5.1.3]) catalyses the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, an essential step in<i>de novo</i>synthesis both of glycine and of purines and deoxythymidine phosphate (the precursors of DNA synthesis) [[cite:PUB00005107]], and important also in the conversion of deoxyuridine monophosphate to deoxythymidine monophosphate. Although DHFR is found ubiquitously in prokaryotes and eukaryotes, and is found in all dividing cells, maintaining levels of fully reduced folate coenzymes, the catabolic steps are still not well understood [[cite:PUB00001361]].</p> <p>Bacterial species possesses distinct DHFR enzymes (based on their pattern of binding diaminoheterocyclic molecules), but mammalian DHFRs are highly similar [[cite:PUB00002379]]. The active site is situated in the N-terminal half of the sequence, which includes a conserved Pro-Trp dipeptide; the tryptophan has been shown [[cite:PUB00002387]] to be involved in the binding of substrate by the enzyme. Its central role in DNA precursor synthesis, coupled with its inhibition by antagonists such as trimethoprim and methotrexate, which are used as anti-bacterial or anti-cancer agents, has made DHFR a target of anticancer chemotherapy. However, resistance has developed against some drugs, as a result of changes in DHFR itself [[cite:PUB00003657]].</p>

This description is obtained from EB-eye REST.

Associated GO terms

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

GO term Namespace Name Definition Relationships
GO:0004146
Molecular function Dihydrofolate reductase activity Catalysis of the reaction: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+.
Subterm of
GO:0006545
Biological process Glycine biosynthetic process The chemical reactions and pathways resulting in the formation of glycine, aminoethanoic acid.
Subterm of
GO:0046654
Biological process Tetrahydrofolate biosynthetic process The chemical reactions and pathways resulting in the formation of tetrahydrofolate, 5,6,7,8-tetrahydrofolic acid, a folate derivative bearing additional hydrogens on the pterin group.
Subterm of
GO:0050661
Molecular function NADP binding Interacting selectively and non-covalently with nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NADP+, or the reduced form, NADPH.
Subterm of
GO:0055114
Biological process Oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
Subterm of

Associated Lotus transcripts 1

Transcript Name Description Predicted domains Domain count
Lj1g3v3381400.1
– PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase-like [Cicer arietinum] gi|502130863|ref|XP_004500795.1|
Domains
17

Co-occuring domains 1

A list of co-occurring predicted domains within the L. japonicus gene space:

Predicted domain Source Observations Saturation (%)
cd00209 CDD 1 100.00