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IPR017925 is a Dihydrofolate reductase conserved site.
<p>Dihydrofolate reductase (DHFR) ([ec:1.5.1.3]) catalyses the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, an essential step in<i>de novo</i>synthesis both of glycine and of purines and deoxythymidine phosphate (the precursors of DNA synthesis) [[cite:PUB00005107]], and important also in the conversion of deoxyuridine monophosphate to deoxythymidine monophosphate. Although DHFR is found ubiquitously in prokaryotes and eukaryotes, and is found in all dividing cells, maintaining levels of fully reduced folate coenzymes, the catabolic steps are still not well understood [[cite:PUB00001361]].</p> <p>Bacterial species possesses distinct DHFR enzymes (based on their pattern of binding diaminoheterocyclic molecules), but mammalian DHFRs are highly similar [[cite:PUB00002379]]. The active site is situated in the N-terminal half of the sequence, which includes a conserved Pro-Trp dipeptide; the tryptophan has been shown [[cite:PUB00002387]] to be involved in the binding of substrate by the enzyme. Its central role in DNA precursor synthesis, coupled with its inhibition by antagonists such as trimethoprim and methotrexate, which are used as anti-bacterial or anti-cancer agents, has made DHFR a target of anticancer chemotherapy. However, resistance has developed against some drugs, as a result of changes in DHFR itself [[cite:PUB00003657]].</p> <p>This entry covers the region in the N-terminal part of the DHFR domain, which includes a conserved Pro-Trp dipeptide; the tryptophan has been shown to be involved in the binding of substrate by the enzyme [[cite:PUB00017878]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Dihydrofolate reductase activity | Catalysis of the reaction: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+. | ||
| Biological process | Oxidation-reduction process | A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase-like [Cicer arietinum] gi|502130863|ref|XP_004500795.1| | 17 | ||
| – | PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase-like [Cicer arietinum] gi|502130863|ref|XP_004500795.1| | 24 | ||
| – | Thymidylate synthase; TAIR: AT2G16370.1 thymidylate synthase 1; Swiss-Prot: sp|P51820|DRTS_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; TrEMBL-Plants: tr|I1K754|I1K754_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; Found in the gene: LotjaGi1g1v0565200 | 25 | ||
| – | Thymidylate synthase; TAIR: AT2G16370.1 thymidylate synthase 1; Swiss-Prot: sp|P51820|DRTS_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; TrEMBL-Plants: tr|I1K753|I1K753_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; Found in the gene: LotjaGi1g1v0565200 | 25 | ||
| – | Thymidylate synthase; TAIR: AT2G16370.1 thymidylate synthase 1; Swiss-Prot: sp|P51820|DRTS_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; TrEMBL-Plants: tr|I1K753|I1K753_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; Found in the gene: LotjaGi1g1v0565200 | 25 | ||
| – | Thymidylate synthase; TAIR: AT4G34570.4 thymidylate synthase 2; Swiss-Prot: sp|P51820|DRTS_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; TrEMBL-Plants: tr|I1K753|I1K753_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; Found in the gene: LotjaGi1g1v0565200 | 24 | ||
| – | Bifunctional dihydrofolate reductase-thymidylate synthase; TAIR: AT2G16370.1 thymidylate synthase 1; Swiss-Prot: sp|P51820|DRTS_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; TrEMBL-Plants: tr|I1K753|I1K753_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; Found in the gene: LotjaGi1g1v0565200 | 17 | ||
| – | Dihydrofolate reductase / thymidylate synthase; TAIR: AT4G34570.1 thymidylate synthase 2; Swiss-Prot: sp|P51820|DRTS_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; TrEMBL-Plants: tr|I1K753|I1K753_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; Found in the gene: LotjaGi1g1v0565200 | 24 | ||
| – | Thymidylate synthase; TAIR: AT2G16370.1 thymidylate synthase 1; Swiss-Prot: sp|P51820|DRTS_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; TrEMBL-Plants: tr|I1K753|I1K753_SOYBN Bifunctional dihydrofolate reductase-thymidylate synthase; Found in the gene: LotjaGi1g1v0565200 | 25 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd00351 | CDD | 1 | 11.11 |