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IPR022450 is a tRNA N6-adenosine threonylcarbamoyltransferase, TsaD.
<p>This entry represents the TsaD protein family that is widely distributed. TsaD and its archaeal homologue Kae1 belong to the Kae1/TsaD family ([interpro:IPR017861]), a conserved protein family with unknown function.</p> <p>This entry includes bacterial TsaD and its homologues, such as Qri7 (localize to the mitochondria) from budding yeast [[cite:PUB00059911]].</p> <p>TsaD (also known as Gcp or YgjD) was originally described as a glycoprotease essential for cell viability [[cite:PUB00059913]] and a critical mediator involved in the modification of cell wall peptidoglycan synthesis and/or cell division [[cite:PUB00059914]]. Gcp is a member of the Kae1/TsaD family, required for the formation of a threonylcarbamoyl group on adenosine at position 37 in tRNAs that read codons beginning with adenine [[cite:PUB00059911]]. YgjD has been renamed as TsaD, and it has been shown that YgjD and proteins YrdC (TsaC), YjeE (TsaE), and YeaZ (TsaB), are necessary and sufficient for t6A biosynthesis in vitro, and may constitute a complex [[cite:PUB00063355]].</p> <p>The first characterised member of the Kae1/TsaD family was annotated as Gcp for O-sialoglycoprotein endopeptidase [[cite:PUB00002152]], but this activity could not be confirmed [[cite:PUB00047751]]. Later, its homologue, Kae1 from Pyrococcus abyssi, has been shown to have DNA-binding properties and apurinic-endonuclease activity [[cite:PUB00047751]]. Members of this family have since been studied in yeast, archaea and bacteria resulting in sometimes conflicting data, several proposed functions and annotations but no definitive characterisation. For instance, some members have been linked to DNA maintenance in bacteria and mitochondria [[cite:PUB00059916]] and transcription regulation and telomere homeostasis in eukaryotes [[cite:PUB00035445], [cite:PUB00035406]], but their function remained unclear. Recent research indicates that this family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine, a universal modification found at position 37 of tRNAs that read codons beginning with adenine [[cite:PUB00059911], [cite:PUB00101388]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | TRNA threonylcarbamoyladenosine modification | The attachment of a carbonyl group and a threonine to the amino group of the adenine residue immediately 3' of the anticodon, in tRNAs that decode ANN codons (where N is any base). | ||
| Molecular function | Metalloendopeptidase activity | Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: probable tRNA threonylcarbamoyladenosine biosynthesis protein osgepl1-like [Glycine max] gi|356506230|ref|XP_003521890.1| | 11 | ||
| – | TRNA N6-adenosine threonylcarbamoyltransferase; TAIR: AT2G45270.1 glycoprotease 1; Swiss-Prot: sp|O22145|OSGP2_ARATH Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial; TrEMBL-Plants: tr|A0A0S3SYJ9|A0A0S3SYJ9_PHAAN Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial; Found in the gene: LotjaGi3g1v0435500 | 12 | ||
| – | TRNA N6-adenosine threonylcarbamoyltransferase; TAIR: AT2G45270.1 glycoprotease 1; Swiss-Prot: sp|O22145|OSGP2_ARATH Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial; TrEMBL-Plants: tr|A0A0S3SYJ9|A0A0S3SYJ9_PHAAN Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial; Found in the gene: LotjaGi3g1v0435500 | 19 | ||
| – | TRNA N6-adenosine threonylcarbamoyltransferase; TAIR: AT2G45270.1 glycoprotease 1; Swiss-Prot: sp|O22145|OSGP2_ARATH Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial; TrEMBL-Plants: tr|A0A0S3SYJ9|A0A0S3SYJ9_PHAAN Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial; Found in the gene: LotjaGi3g1v0435500 | 18 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| TIGR03723 | TIGRFAM | 1 | 25.00 |