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IPR034680 is a tRNA N6-adenosine threonylcarbamoyltransferase Kae1, archaeal/eukaryotic.
<p>The KEOPS/EKC complex is a tRNA modification complex involved in the biosynthesis of N6-threonylcarbamoyladenosine (t6A), a universally conserved tRNA modification found on ANN-codon recognizing tRNAs [[cite:PUB00084172]]. In archaea and eukaryotes, KEOPS is composed of Kae1, a highly conserved protein present in bacteria, archaea and eukaryotes, kinase PRPK/Bud32, and three additional polypeptides (Pcc1, Cgi121, and Gon7) [[cite:PUB00059910], [cite:PUB00084171]].</p> <p>The first characterised member of the Kae1/TsaD family was annotated as Gcp for O-sialoglycoprotein endopeptidase [[cite:PUB00002152]], but this activity could not be confirmed [[cite:PUB00047751]]. Later, its homologue, Kae1 from Pyrococcus abyssi, has been shown to have DNA-binding properties and apurinic-endonuclease activity [[cite:PUB00047751]]. Members of this family have since been studied in yeast, archaea and bacteria resulting in sometimes conflicting data, several proposed functions and annotations but no definitive characterisation. For instance, some members have been linked to DNA maintenance in bacteria and mitochondria [[cite:PUB00059916]] and transcription regulation and telomere homeostasis in eukaryotes [[cite:PUB00035445], [cite:PUB00035406]], but their function remained unclear. Recent research indicates that this family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine, a universal modification found at position 37 of tRNAs that read codons beginning with adenine [[cite:PUB00059911], [cite:PUB00101388]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Cellular component | EKC/KEOPS complex | A protein complex involved in t6A tRNA modification; originally proposed to be involved in transcription as well as promoting telomere uncapping and telomere elongation. For example, in Saccharomyces cerevisiae the complex contains Bud32p, Kae1p, Gon7p, Cgi121p, and Pcc1p. | ||
| Biological process | TRNA threonylcarbamoyladenosine modification | The attachment of a carbonyl group and a threonine to the amino group of the adenine residue immediately 3' of the anticodon, in tRNAs that decode ANN codons (where N is any base). |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | TRNA N6-adenosine threonylcarbamoyltransferase; TAIR: AT4G22720.1 Actin-like ATPase superfamily protein; Swiss-Prot: sp|Q9WVS2|OSGEP_RAT Probable tRNA N6-adenosine threonylcarbamoyltransferase; TrEMBL-Plants: tr|I1NCH1|I1NCH1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0788800 | 13 | ||
| – | TRNA N6-adenosine threonylcarbamoyltransferase; TAIR: AT4G22720.1 Actin-like ATPase superfamily protein; Swiss-Prot: sp|Q9WVS2|OSGEP_RAT Probable tRNA N6-adenosine threonylcarbamoyltransferase; TrEMBL-Plants: tr|I1NCH1|I1NCH1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0788800 | 13 | ||
| – | TRNA N6-adenosine threonylcarbamoyltransferase; TAIR: AT4G22720.1 Actin-like ATPase superfamily protein; Swiss-Prot: sp|Q9WVS2|OSGEP_RAT Probable tRNA N6-adenosine threonylcarbamoyltransferase; TrEMBL-Plants: tr|I1NCH1|I1NCH1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0788800 | 11 | ||
| – | TRNA N6-adenosine threonylcarbamoyltransferase; TAIR: AT4G22720.1 Actin-like ATPase superfamily protein; Swiss-Prot: sp|Q9WVS2|OSGEP_RAT Probable tRNA N6-adenosine threonylcarbamoyltransferase; TrEMBL-Plants: tr|I1NCH1|I1NCH1_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0788800 | 13 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd00012 | CDD | 1 | 25.00 |