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Field | Value |
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Namespace | Cellular component |
Short description | Oligosaccharyltransferase complex |
Full defintion | A protein complex that is found in the endoplasmic reticulum membrane of eukaryotes and transfers lipid-linked oligosaccharide precursor to asparagine residues on nascent proteins. In yeast, the complex includes at least nine different subunits, whereas in mammalian cells at least three different forms of the complex have been detected. |
Subterm of |
The relationship of GO:0008250 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0008250, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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ISBN | Essentials of Glycobiology · , 1999 · 680 pages Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans. |
PMID | Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits. Biochemistry. 2005 Apr 26; 44 (16): 5982–92.PMID: 15835887 Oligosaccharyltransferase (OST) catalyzes the cotranslational transfer of high-mannose sugars to nascent polypeptides during N-linked glycosylation in the rough endoplasmic reticulum lumen. Nine OST subunits have been identified in yeast. However, the composition and organization of mammalian OST remain unclear. Using two-dimensional Blue Native polyacrylamide gel electrophoresis/sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry, we now demonstrate that mammalian OST can be isolated from solubilized, actively engaged ribosomes as multiple distinct protein complexes that range in size from approximately 500 to 700 kDa. These complexes exhibit different ribosome affinities and subunit compositions. The major complex, OSTC(I), had an apparent size of approximately 500 kDa and was readily released from ribosome translocon complexes after puromycin treatment under physiological salt conditions. Two additional complexes were released only after treatment with high salt: OSTC(II) ( approximately 600 kDa) and OSTC(III) ( approximately 700 kDa). Both remained stably associated with heterotrimeric Sec61alphabetagamma, while OSTC(III) also contained the tetrameric TRAP complex. All known mammalian OST subunits (STT3-A, ribophorin I, ribophorin II, OST48, and DAD1) were present in all complexes. In addition, two previously uncharacterized proteins were also copurified with OST. Mass spectrometry identified a 17 kDa protein as DC2 which is weakly homologous to the C-terminal half of yeast Ost3p and Ost6p. The second protein (14 kDa) was tentatively identified as keratinocyte-associated protein 2 (KCP2) and has no previously known function. Our results identify two potential new subunits of mammalian OST and demonstrate a remarkable heterogeneity in OST composition that may reflect a means for controlling nascent chain glycosylation. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
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– | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2-like [Glycine max] gi|356505035|ref|XP_003521298.1| | 3 | ||
– | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2-like [Glycine max] gi|356537059|ref|XP_003537048.1| | 3 | ||
– | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; TAIR: AT1G32210.1 Defender against death (DAD family) protein; Swiss-Prot: sp|Q39080|DAD1_ARATH Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; TrEMBL-Plants: tr|A0A1J7GF25|A0A1J7GF25_LUPAN Uncharacterized protein; Found in the gene: LotjaGi2g1v0337000 | 2 | ||
– | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; TAIR: AT1G32210.1 Defender against death (DAD family) protein; Swiss-Prot: sp|Q9ZWQ7|DAD1_CITUN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; TrEMBL-Plants: tr|V7CLM9|V7CLM9_PHAVU Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Found in the gene: LotjaGi4g1v0247400 | 2 | ||
– | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; TAIR: AT4G21150.1 ribophorin II (RPN2) family protein; Swiss-Prot: sp|Q93Z16|RPN2_ARATH Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; TrEMBL-Plants: tr|V7BJX8|V7BJX8_PHAVU Uncharacterized protein; Found in the gene: LotjaGi5g1v0157700 | 3 | ||
– | Oligosaccharyltransferase complex subunit OSTC-like protein; TAIR: AT4G29870.1 Oligosaccharyltransferase complex/magnesium transporter family protein; Swiss-Prot: sp|Q5M9B7|OSTCB_XENLA Oligosaccharyltransferase complex subunit ostc-B; TrEMBL-Plants: tr|G7IIC1|G7IIC1_MEDTR Oligosaccharyltransferase complex subunit OSTC-like protein; Found in the gene: LotjaGi6g1v0233700 | 2 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Cellular component | Oligosaccharyltransferase complex | 1 | 16.67 |