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GO:0017108

View this GO term in other databases

Overview

Field Value
Namespace Molecular function
Short description 5'-flap endonuclease activity
Full defintion Catalysis of the cleavage of a 5' flap structure in DNA, but not other DNA structures; processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis.
Subterm of

Relationships

The relationship of GO:0017108 with other GO terms.

Relationship type GO terms
Is a
Regulates n.a.
Part of n.a.
Positively regulates n.a.
Negatively regulates n.a.

Ancestor tree

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Additional data

This table contains additional metadata associated with the GO entry's definition field.

Field Value
PMID
Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity.Hosfield DJ, Mol CD, Shen B, Tainer JA
Cell. 1998 Oct 2; 95 (1): 135–46.PMID: 9778254

Flap endonuclease (FEN-1) removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The crystal structure of Pyrococcus furiosus FEN-1, active-site metal ions, and mutational information indicate interactions for the single- and double-stranded portions of the flap DNA substrate and identify an unusual DNA-binding motif. The enzyme's active-site structure suggests that DNA binding induces FEN-1 to clamp onto the cleavage junction to form the productive complex. The conserved FEN-1 C terminus binds proliferating cell nuclear antigen (PCNA) and positions FEN-1 to act primarily as an exonuclease in DNA replication, in contrast to its endonuclease activity in DNA repair. FEN-1 mutations altering PCNA binding should reduce activity during replication, likely causing DNA repeat expansions as seen in some cancers and genetic diseases.

Associated Lotus transcripts 7

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .

Transcript Name Description GO terms GO count
Lj0g3v0072759.1
– PREDICTED: structure-specific endonuclease subunit SLX1-like [Glycine max] gi|356537639|ref|XP_003537333.1|
GO terms
3
Lj0g3v0281719.1
– PREDICTED: DNA replication ATP-dependent helicase/nuclease DNA2-like [Cicer arietinum] gi|502169493|ref|XP_004514619.1|
GO terms
3
Lj0g3v0287199.1
– DNA replication helicase dna2 isoform 2 [Theobroma cacao] gi|508725636|gb|EOY17533.1|
GO terms
3
LotjaGi1g1v0604000.1
– DNA replication helicase dna2, putative; TAIR: AT1G08840.4 DNA replication helicase; Swiss-Prot: sp|Q8QHA5|DNA2_XENLA DNA replication ATP-dependent helicase/nuclease DNA2; TrEMBL-Plants: tr|A0A072TZB7|A0A072TZB7_MEDTR DNA replication ATP-dependent helicase Dna2; Found in the gene: LotjaGi1g1v0604000
GO terms
3
LotjaGi1g1v0604000.2
– DNA replication helicase dna2, putative; TAIR: AT1G08840.2 DNA replication helicase; Swiss-Prot: sp|Q8QHA5|DNA2_XENLA DNA replication ATP-dependent helicase/nuclease DNA2; TrEMBL-Plants: tr|K7KYE7|K7KYE7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0604000
GO terms
3
LotjaGi1g1v0604000.3
– DNA replication helicase dna2, putative; TAIR: AT1G08840.3 DNA replication helicase; Swiss-Prot: sp|Q8QHA5|DNA2_XENLA DNA replication ATP-dependent helicase/nuclease DNA2; TrEMBL-Plants: tr|K7KYE7|K7KYE7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0604000
GO terms
3
LotjaGi6g1v0104600.1
– Structure-specific endonuclease subunit SLX1 homolog; TAIR: AT2G30350.2 Excinuclease ABC, C subunit, N-terminal; Swiss-Prot: sp|Q8BX32|SLX1_MOUSE Structure-specific endonuclease subunit SLX1; TrEMBL-Plants: tr|I1LLV5|I1LLV5_SOYBN Structure-specific endonuclease subunit SLX1 homolog; Found in the gene: LotjaGi6g1v0104600
GO terms
3

Co-occuring GO terms 1

A list of co-occurring GO terms within the L. japonicus gene space:

GO term Namespace Name Observations Saturation (%)
GO:0033557
Cellular component Slx1-Slx4 complex 1 14.29