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Field | Value |
---|---|
Namespace | Molecular function |
Short description | NEDD8 activating enzyme activity |
Full defintion | Catalysis of the initiation of the NEDD8 (RUB1) conjugation cascade. |
Subterm of |
The relationship of GO:0019781 with other GO terms.
Relationship type | GO terms |
---|---|
Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0019781, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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PMID | Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8. Nature. 2003 Mar 20; 422 (6929): 330–4.PMID: 12646924 Post-translational modification by ubiquitin-like proteins (Ublps) is an essential cellular regulatory mechanism. The Ublp NEDD8 regulates cell division, signalling and embryogenesis. Ublps are conjugated to their targets by the sequential action of E1, E2 and often E3 enzymes. Each Ublp has a dedicated E1, or activating enzyme, that initiates its conjugation cascade. First, E1 associates with the Ublp and catalyses adenylation of the carboxy terminus of the Ublp. Second, E1 forms a thioester between its catalytic cysteine and the Ublp. Next, E1 is loaded with a second Ublp molecule, adenylating the C terminus of this second Ublp while still carrying the first thioester-bound Ublp. Last, E1 binds E2 and promotes Ublp transfer to the catalytic cysteine of E2. We report here the structure and mutational analysis of human APPBP1-UBA3, the heterodimeric E1 enzyme for NEDD8 (ref. 11). Each E1 activity is specified by a domain: an adenylation domain resembling bacterial adenylating enzymes, an E1-specific domain organized around the catalytic cysteine, and a domain involved in E2 recognition resembling ubiquitin. The domains are arranged around two clefts that coordinate protein and nucleotide binding so that each of E1's reactions drives the next, in an assembly-line fashion. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: NEDD8-activating enzyme E1 regulatory subunit-like [Glycine max] gi|356517638|ref|XP_003527494.1| | 2 | ||
– | PREDICTED: NEDD8-activating enzyme E1 catalytic subunit-like [Cicer arietinum] gi|502149911|ref|XP_004507708.1| | 2 | ||
– | NEDD8-activating enzyme E1 regulatory subunit; TAIR: AT1G05180.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|P42744|AXR1_ARATH NEDD8-activating enzyme E1 regulatory subunit AXR1; TrEMBL-Plants: tr|I3SW23|I3SW23_LOTJA NEDD8-activating enzyme E1 regulatory subunit; Found in the gene: LotjaGi3g1v0466500 | 2 | ||
– | NEDD8-activating enzyme E1 regulatory subunit; TAIR: AT1G05180.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|P42744|AXR1_ARATH NEDD8-activating enzyme E1 regulatory subunit AXR1; TrEMBL-Plants: tr|I3SW23|I3SW23_LOTJA NEDD8-activating enzyme E1 regulatory subunit; Found in the gene: LotjaGi3g1v0466500 | 2 | ||
– | NEDD8-activating enzyme E1 catalytic subunit; TAIR: AT5G19180.1 E1 C-terminal related 1; Swiss-Prot: sp|O65041|UBA3_ARATH NEDD8-activating enzyme E1 catalytic subunit; TrEMBL-Plants: tr|I1KNA2|I1KNA2_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0352900 | 2 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Biological process | Protein neddylation | 1 | 20.00 |