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Field | Value |
---|---|
Namespace | Cellular component |
Short description | Ribonuclease H2 complex |
Full defintion | A protein complex that possesses ribonuclease H activity, in which the catalytic subunit is a member of the RNase H2 (or HII) class. For example, in Saccharomyces the complex contains Rnh201p, Rnh202p and Rnh203p. |
Subterm of |
The relationship of GO:0032299 with other GO terms.
Relationship type | GO terms |
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Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0032299, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
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GOC | mah |
PMID | RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. Nucleic Acids Res. 2004; 32 (2): 407–14.PMID: 14734815 The composition of RNase H2 has been a long-standing problem. Whereas bacterial and archaeal RNases H2 are active as single polypeptides, the Saccharomyces cerevisiae homolog, Rnh2Ap, when expressed in Escherichia coli, fails to produce an active RNase H2. By affinity chromatography purification and identification of polypeptides associated with a tagged S.cerevisiae Rnh2Ap, we obtained a complex of three proteins [Rnh2Ap (Rnh201p), Ydr279p (Rnh202p) and Ylr154p (Rnh203p)] that together are necessary and sufficient for RNase H2 activity [correction]. Deletion of the gene encoding any one of the proteins or mutations in the catalytic site in Rnh2A led to loss of RNase H2 activity. Even when S.cerevisiae RNase H2 is catalytically compromised, it still exhibits a preference for cleavage of the phosphodiester bond on the 5' side of a ribonucleotide-deoxyribonucleotide sequence in substrates mimicking RNA-primed Okazaki fragments or a single ribonucleotide embedded in a duplex DNA. Interestingly, Ydr279p and Ylr154p have homologous proteins only in closely related species. The multisubunit nature of S.cerevisiae RNase H2 may be important both for structural purposes and to provide a means of interacting with other proteins involved in DNA replication/repair and transcription. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: ribonuclease H2 subunit C-like [Cicer arietinum] gi|502150052|ref|XP_004507766.1| | 2 | ||
– | Ribonuclease H2 subunit B; TAIR: AT4G20325.1 ribonuclease H2 subunit B; Swiss-Prot: sp|Q5HZP1|RNH2B_XENLA Ribonuclease H2 subunit B; TrEMBL-Plants: tr|I1K961|I1K961_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0396700 | 1 | ||
– | Ribonuclease H2 subunit C; TAIR: AT2G39440.1 ribonuclease H2 subunit C-like protein; Swiss-Prot: sp|Q8TDP1|RNH2C_HUMAN Ribonuclease H2 subunit C; TrEMBL-Plants: tr|I3SJ55|I3SJ55_LOTJA Uncharacterized protein; Found in the gene: LotjaGi4g1v0290800 | 2 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Cellular component | Ribonuclease H2 complex | 1 | 33.33 |