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| Field | Value |
|---|---|
| Namespace | Cellular component |
| Short description | FACT complex |
| Full defintion | An abundant nuclear complex, which was originally identified in mammalian systems as a factor required for transcription elongation on chromatin templates. The FACT complex has been shown to destablilize the interaction between the H2A/H2B dimer and the H3/H4 tetramer of the nucleosome, thus reorganizing the structure of the nucleosome. In this way, the FACT complex may play a role in DNA replication and other processes that traverse the chromatin, as well as in transcription elongation. FACT is composed of two proteins that are evolutionarily conserved in all eukaryotes and homologous to mammalian Spt16 and SSRP1. In metazoans, the SSRP1 homolog contains an HMG domain; however in fungi and protists, it does not. For example, in S. cerevisiae the Pob3 protein is homologous to SSRP1, but lacks the HMG chromatin binding domain. Instead, the yFACT complex of Spt16p and Pob3p, binds to nucleosomes where multiple copies of the HMG-domain containing protein Nhp6p have already bound, but Nhp6p does not form a stable complex with the Spt16p/Pob3p heterodimer. |
| Subterm of |
The relationship of GO:0035101 with other GO terms.
| Relationship type | GO terms |
|---|---|
| Is a | |
| Regulates | n.a. |
| Part of | n.a. |
| Positively regulates | n.a. |
| Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0035101, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
| Field | Value |
|---|---|
| GOC | krc |
| PMID | The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. Mol Cell. 2006 May 5; 22 (3): 363–74.PMID: 16678108 We report the crystal structure of the middle domain of the Pob3 subunit (Pob3-M) of S. cerevisiae FACT (yFACT, facilitates chromatin transcription), which unexpectedly adopts an unusual double pleckstrin homology (PH) architecture. A mutation within a conserved surface cluster in this domain causes a defect in DNA replication that is suppressed by mutation of replication protein A (RPA). The nucleosome reorganizer yFACT therefore interacts in a physiologically important way with the central single-strand DNA (ssDNA) binding factor RPA to promote a step in DNA replication. Purified yFACT and RPA display a weak direct physical interaction, although the genetic suppression is not explained by simple changes in affinity between the purified proteins. Further genetic analysis suggests that coordinated function by yFACT and RPA is important during nucleosome deposition. These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| Transcript | Name | Description | GO terms | GO count |
|---|---|---|---|---|
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7H1L5|A0A1J7H1L5_LUPAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0481300 | 1 | ||
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7H1L5|A0A1J7H1L5_LUPAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0481300 | 1 | ||
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7H1L5|A0A1J7H1L5_LUPAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0051400 | 1 | ||
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7H1L5|A0A1J7H1L5_LUPAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0051400 | 1 | ||
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7H1L5|A0A1J7H1L5_LUPAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0051400 | 1 | ||
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7IIX4|A0A1J7IIX4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0051500 | 1 | ||
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7IIX4|A0A1J7IIX4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0051500 | 1 | ||
| – | FACT complex subunit SPT16; TAIR: AT4G10710.1 global transcription factor C; Swiss-Prot: sp|O82491|SPT16_ARATH FACT complex subunit SPT16; TrEMBL-Plants: tr|A0A1J7IIX4|A0A1J7IIX4_LUPAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0051500 | 1 |
A list of co-occurring GO terms within the L. japonicus gene space:
| GO term | Namespace | Name | Observations | Saturation (%) |
|---|---|---|---|---|
| Cellular component | FACT complex | 1 | 12.50 |