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IPR002301 is a Isoleucine-tRNA ligase.
<p>Isoleucine-tRNA ligase (also known as Isoleucyl-tRNA synthetase)([ec:6.1.1.5]) is an alpha monomer that belongs to class Ia. The enzyme, isoleucine-tRNA ligase, activates not only the cognate substrate L-isoleucine but also the minimally distinct L-valine in the first, aminoacylation step. Then, in a second, "editing" step, the ligase itself rapidly hydrolyses only the valylated products [[cite:PUB00006404], [cite:PUB00006454]] as shown from the crystal structures.</p> <p>The aminoacyl-tRNA synthetases (also known as aminoacyl-tRNA ligases) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction [[cite:PUB00079872], [cite:PUB00079873]]. These proteins differ widely in size and oligomeric state, and have limited sequence homology [[cite:PUB00007191]]. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric [[cite:PUB00006477]]. Class II aminoacyl-tRNA synthetases share an anti-parallel β-sheet fold flanked by α-helices [[cite:PUB00000386]], and are mostly dimeric or multimeric, containing at least three conserved regions [[cite:PUB00000723], [cite:PUB00005365], [cite:PUB00004391]]. However, tRNA binding involves an α-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan, valine, and some lysine synthetases (non-eukaryotic group) belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, phenylalanine, proline, serine, threonine, and some lysine synthetases (non-archaeal group), belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c [[cite:PUB00007363]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Nucleotide binding | Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. | ||
| Molecular function | Isoleucine-tRNA ligase activity | Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+). | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Biological process | Isoleucyl-tRNA aminoacylation | The process of coupling isoleucine to isoleucyl-tRNA, catalyzed by isoleucyl-tRNA synthetase. The isoleucyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2'-OH group of a isoleucine-accetping tRNA. The 2'-O-aminoacyl-tRNA will ultimately migrate to the 3' position via transesterification. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: isoleucine--tRNA ligase, cytoplasmic-like [Cicer arietinum] gi|502144299|ref|XP_004505648.1| | 24 | ||
| – | PREDICTED: isoleucine--tRNA ligase-like [Cicer arietinum] gi|502085158|ref|XP_004487851.1| | 28 | ||
| – | Isoleucine-tRNA ligase; TAIR: AT4G10320.1 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|F4JLM5|SYIC_ARATH Isoleucine--tRNA ligase, cytoplasmic; TrEMBL-Plants: tr|A0A0S3SEB7|A0A0S3SEB7_PHAAN Uncharacterized protein; Found in the gene: LotjaGi3g1v0535700 | 23 | ||
| – | Isoleucine-tRNA ligase; TAIR: AT5G49030.3 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|Q8RXK8|SYIM_ARATH Isoleucine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A072V611|A0A072V611_MEDTR Isoleucine-tRNA ligase; Found in the gene: LotjaGi6g1v0249900 | 29 | ||
| – | Isoleucine-tRNA ligase; TAIR: AT5G49030.3 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|Q8RXK8|SYIM_ARATH Isoleucine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A072V611|A0A072V611_MEDTR Isoleucine-tRNA ligase; Found in the gene: LotjaGi6g1v0249900 | 28 | ||
| – | Isoleucine-tRNA ligase; TAIR: AT5G49030.3 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|Q8RXK8|SYIM_ARATH Isoleucine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A072V611|A0A072V611_MEDTR Isoleucine-tRNA ligase; Found in the gene: LotjaGi6g1v0249900 | 27 | ||
| – | Isoleucine-tRNA ligase; TAIR: AT5G49030.3 tRNA synthetase class I (I, L, M and V) family protein; Swiss-Prot: sp|Q8RXK8|SYIM_ARATH Isoleucine--tRNA ligase, chloroplastic/mitochondrial; TrEMBL-Plants: tr|A0A072V611|A0A072V611_MEDTR Isoleucine-tRNA ligase; Found in the gene: LotjaGi6g1v0249900 | 29 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 14.29 |