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IPR002745 is a Phosphotransferase KptA/Tpt1.
<p>This entry includes Tpt1 and its homologues from all domains of life. Tpt1 was first discovered as an essential component of the fungal tRNA splicing pathway, which characteristically generates a 2'-PO4, 3'-5' phosphodiester splice junction during the tRNA ligation reaction [[cite:PUB00094147]]. It is an enzyme that catalyzes the transfer of an internal RNA 2'-monophosphate (2'-PO4) to NAD+ to form a 2'-OH RNA, ADP-ribose 1"-2" cyclic phosphate, and nicotinamide [[cite:PUB00094142]].</p> <p>Interestingly, many Tpt1 homologues have no obvious need for an RNA 2'-phosphotransferase because: (i) they lack tRNA introns; and/or (ii) they are not known to have an enzymatic pathway that generates 2'-PO4, 3'-5' phosphodiester RNA structures. There are more evidence showing that Tpt1 homologues are not limited to the canonical activity of Tpt1 healing the 2'-PO4, 3'-5' phosphodiester RNA splice junction formed during fungal and plant tRNA splicing [[cite:PUB00094148]]. Instead, the ADP-ribosyl transfer to a phosphorylated substrate is the unifying mechanistic feature of Tpt1-catalyzed reactions. For instance, some Tpt1 homologues have been shown to catalyze the transfer of ADP-ribose from NAD+ to a 5'-monophosphate end of RNA or DNA to install a 5'-phospho-ADP-ribose cap structure [[cite:PUB00094146]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | TRNA splicing, via endonucleolytic cleavage and ligation | Splicing of tRNA substrates via recognition of the folded RNA structure that brings the 5' and 3' splice sites into proximity and cleavage of the RNA at both the 3' and 5' splice sites by an endonucleolytic mechanism, followed by ligation of the exons. | ||
| Molecular function | Transferase activity, transferring phosphorus-containing groups | Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor). |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: tRNA 2'-phosphotransferase 1-like isoform X1 [Cicer arietinum] gi|502146539|ref|XP_004506497.1| | 7 | ||
| – | RNA 2'-phosphotransferase; TAIR: AT2G45330.2 RNA 2'-phosphotransferase, Tpt1 / KptA family; Swiss-Prot: sp|Q5EAR5|TRPT1_DANRE tRNA 2'-phosphotransferase 1; TrEMBL-Plants: tr|I3SLL1|I3SLL1_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0432800 | 10 | ||
| – | RNA 2'-phosphotransferase isoform 1; TAIR: AT2G45330.1 RNA 2'-phosphotransferase, Tpt1 / KptA family; Swiss-Prot: sp|O14045|TPT1_SCHPO Putative tRNA 2'-phosphotransferase; TrEMBL-Plants: tr|I3S0W1|I3S0W1_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0432800 | 6 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| SSF56399 | SUPERFAMILY | 1 | 33.33 |