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IPR003180 is a Methylpurine-DNA glycosylase.
<p>Methylpurine-DNA glycosylase (MPG, or alkyladenine DNA glycosylase (AAG)) is a base excision-repair protein, catalyzing the first step in base excision repair by cleaving damaged DNA bases within double-stranded DNA to produce an abasic site. MPG bends DNA by intercalating between the base pairs, causing the damaged base to flip out of the double helix and into the enzyme active site for cleavage. It is responsible for the hydrolysis of the deoxyribose N-glycosidic bond, excising 3-methyladenine and 3-methylguanine from damaged DNA [[cite:PUB00043337], [cite:PUB00079895], [cite:PUB00016253], [cite:PUB00020209], [cite:PUB00014119], [cite:PUB00079896], [cite:PUB00079897], [cite:PUB00079898], [cite:PUB00079899], [cite:PUB00079900], [cite:PUB00079901]]. Its action is induced by alkylating chemotherapeutics, as well as deaminated and lipid peroxidation-induced purine adducts [[cite:PUB00043338]]. MPG without an N-terminal extension excises hypoxanthine with one-third of the efficiency of full-length MPG under similar conditions, suggesting that is function may largely be attributable to the N-terminal extension [[cite:PUB00043339]].</p> <p>Although AAG represents one of six DNA glycosylase classes, it lacks the helix-hairpin-helix active site motif associated with other base excision repair glycosylases and is structurally distinct from them.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). | ||
| Molecular function | Alkylbase DNA N-glycosylase activity | Catalysis of the reaction: DNA with alkylated base + H2O = DNA with abasic site + alkylated base. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar to remove an alkylated base, leaving an apyrimidinic or apurinic site. | ||
| Biological process | Base-excision repair | In base excision repair, an altered base is removed by a DNA glycosylase enzyme, followed by excision of the resulting sugar phosphate. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: DNA-3-methyladenine glycosylase-like isoform X1 [Cicer arietinum] gi|502109262|ref|XP_004493604.1| | 8 | ||
| – | DNA-3-methyladenine glycosylase; TAIR: AT3G12040.1 DNA-3-methyladenine glycosylase (MAG); Swiss-Prot: sp|Q39147|3MG_ARATH DNA-3-methyladenine glycosylase; TrEMBL-Plants: tr|I1N9A6|I1N9A6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0279000 | 11 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 50.00 |