Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR003358 is a tRNA (guanine-N-7) methyltransferase, Trmb type.
<p>This entry represents tRNA (guanine-N-7) methyltransferase ([ec:2.1.1.33]), which catalyses the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Capping of the pre-mRNA 5' end by addition a monomethylated guanosine cap (m(7)G) is an essential and the earliest modification in the biogenesis of mRNA [[cite:PUB00044795]]. The reaction is catalysed by three enzymes: triphosphatase, guanylyltransferase, and tRNA (guanine-N-7) methyltransferase [[cite:PUB00017356], [cite:PUB00043301]]. This entry includes Bacillus subtilis TrmB, which contains a unique variant of the Rossmann-fold methyltransferase (RFM) structure, with the N-terminal helix folded on the opposite site of the catalytic domain [[cite:PUB00040616]].</p> <p>Methyltransferases (EC [ec:2.1.1.-]) constitute an important class of enzymes present in every life form. They transfer a methyl group most frequently from S-adenosyl L-methionine (SAM or AdoMet) to a nucleophilic acceptor such as oxygen leading to S-adenosyl-L-homocysteine (AdoHcy) and a methylated molecule [[cite:PUB00057957], [cite:PUB00057958], [cite:PUB00054125]]. All these enzymes have in common a conserved region of about 130 amino acid residues that allow them to bind SAM [[cite:PUB00006319]]. The substrates that are methylated by these enzymes cover virtually every kind of biomolecules ranging from small molecules, to lipids, proteins and nucleic acids [[cite:PUB00057957], [cite:PUB00057958], [cite:PUB00006319]]. Methyltransferase are therefore involved in many essential cellular processes including biosynthesis, signal transduction, protein repair, chromatin regulation and gene silencing [[cite:PUB00057957], [cite:PUB00057958], [cite:PUB00054125]]. More than 230 families of methyltransferases have been described so far, of which more than 220 use SAM as the methyl donor.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | TRNA modification | The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically. | ||
| Molecular function | TRNA (guanine-N7-)-methyltransferase activity | Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N7-methylguanine. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: phosphoglycerate kinase-like [Glycine max] gi|356551092|ref|XP_003543912.1| | 16 | ||
| – | PREDICTED: tRNA (guanine-N(7)-)-methyltransferase-like [Glycine max] gi|356503164|ref|XP_003520381.1| | 9 | ||
| – | PREDICTED: tRNA (guanine-N(7)-)-methyltransferase-like [Glycine max] gi|356503164|ref|XP_003520381.1| | 16 | ||
| – | TRNA (guanine-N(7)-)-methyltransferase; TAIR: AT5G24840.1 tRNA (guanine-N-7) methyltransferase; Swiss-Prot: sp|Q8GXB7|TRMB_ARATH tRNA (guanine-N(7)-)-methyltransferase; TrEMBL-Plants: tr|I3SH34|I3SH34_LOTJA tRNA (guanine-N(7)-)-methyltransferase; Found in the gene: LotjaGi1g1v0398300 | 12 | ||
| – | Phosphoglycerate kinase; TAIR: AT3G12780.1 phosphoglycerate kinase 1; Swiss-Prot: sp|Q31P73|PGK_SYNE7 Phosphoglycerate kinase; TrEMBL-Plants: tr|K7LXU3|K7LXU3_SOYBN Phosphoglycerate kinase; Found in the gene: LotjaGi2g1v0074400 | 15 | ||
| – | Phosphoglycerate kinase; TAIR: AT3G12780.1 phosphoglycerate kinase 1; Swiss-Prot: sp|Q31P73|PGK_SYNE7 Phosphoglycerate kinase; TrEMBL-Plants: tr|K7LXU3|K7LXU3_SOYBN Phosphoglycerate kinase; Found in the gene: LotjaGi2g1v0074400 | 15 | ||
| – | Phosphoglycerate kinase; TAIR: AT3G12780.1 phosphoglycerate kinase 1; Swiss-Prot: sp|Q31P73|PGK_SYNE7 Phosphoglycerate kinase; TrEMBL-Plants: tr|K7LXU9|K7LXU9_SOYBN Phosphoglycerate kinase; Found in the gene: LotjaGi2g1v0074400 | 15 | ||
| – | TRNA (Guanine-N(7)-)-methyltransferase; TAIR: AT5G17660.1 tRNA (guanine-N-7) methyltransferase; Swiss-Prot: sp|Q2JJQ0|TRMB_SYNJB tRNA (guanine-N(7)-)-methyltransferase; TrEMBL-Plants: tr|I1JMG9|I1JMG9_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0073800 | 16 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd02440 | CDD | 1 | 12.50 |