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IPR004143 is a Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain.
<p>Biotin and lipoic acid are the covalently bound cofactors of various multicomponent enzyme complexes that catalyse key metabolic reactions. In these enzymes complexes, biotin and lipoic acid are attached via amide linkage through their carboxyl group and the ε-amino group of a specific lysine residue of a protein module known respectively as the biotinyl and the lipoyl domain. Covalent attachment of biotin and lipoic acid to these enzyme complexes occurs post-translationally, and it is mediated by biotinylating and lipoylating protein enzymes, which specifically recognise the biotinyl and lipoyl domains, ensuring their correct post-translational modification. Lipoylating and biotinylating enzymes are evolutionarily related protein families containing a homologous catalytic module [[cite:PUB00019228]].</p> <p>Amino acid sequence conservation between the catalytic modules of biotinyl protein ligases (BPLs) and lipoyl protein ligases (LPLs) is very low, and mainly affects residues that are important for the scaffold of the structure, such as those contributing to the hydrophobic core. Despite the poor overall sequence similarity, a single lysine residue is strictly conserved in all LPL and BPL sequences. This lysine residue is likely to bind specifically to the carbonyl oxygen of the carboxyl group of biotin or at the end of the hydrogen- carbon tail of the lipoyl moiety [[cite:PUB00019228]]. The BPL/LPL catalytic domain contains a seven-stranded mixed β-sheet on one side and four α-helices on the other side [[cite:PUB00038115]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | Cellular protein modification process | The covalent alteration of one or more amino acids occurring in proteins, peptides and nascent polypeptides (co-translational, post-translational modifications) occurring at the level of an individual cell. Includes the modification of charged tRNAs that are destined to occur in a protein (pre-translation modification). |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: biotin--protein ligase-like [Cicer arietinum] gi|502130276|ref|XP_004500594.1| | 7 | ||
| – | Holocarboxylase synthetase hcs2 [Medicago truncatula] gi|357461633|ref|XP_003601098.1| | 12 | ||
| – | PREDICTED: plastidial lipoyltransferase 2-like [Glycine max] gi|356509700|ref|XP_003523584.1| | 12 | ||
| – | Lipoate protein ligase-like protein [Medicago truncatula] gi|357501933|ref|XP_003621255.1| | 6 | ||
| – | PREDICTED: octanoyltransferase-like [Cicer arietinum] gi|502120173|ref|XP_004496872.1| | 14 | ||
| – | Biotin holocarboxylase synthetase 1-like protein; TAIR: AT2G25710.1 holocarboxylase synthase 1; Swiss-Prot: sp|Q9SL92|HCS1_ARATH Biotin--protein ligase 1, chloroplastic; TrEMBL-Plants: tr|G7J3L5|G7J3L5_MEDTR Biotin holocarboxylase synthetase, putative; Found in the gene: LotjaGi1g1v0254100 | 12 | ||
| – | Lipoate-protein ligase A; TAIR: AT3G29010.1 Biotin/lipoate A/B protein ligase family; Swiss-Prot: sp|P47512|LPLA_MYCGE Probable lipoate-protein ligase A; TrEMBL-Plants: tr|V7B115|V7B115_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0504900 | 7 | ||
| – | Lipoate-protein ligase A; TAIR: AT3G29010.4 Biotin/lipoate A/B protein ligase family; Swiss-Prot: sp|O13629|LPLA_SCHPO Putative lipoate-protein ligase A; TrEMBL-Plants: tr|K7LFQ5|K7LFQ5_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0504900 | 10 | ||
| – | Lipoate-protein ligase A; TAIR: AT3G29010.1 Biotin/lipoate A/B protein ligase family; Swiss-Prot: sp|P47512|LPLA_MYCGE Probable lipoate-protein ligase A; TrEMBL-Plants: tr|V7B115|V7B115_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0504900 | 6 | ||
| – | Lipoate-protein ligase A; TAIR: AT3G29010.1 Biotin/lipoate A/B protein ligase family; Swiss-Prot: sp|P47512|LPLA_MYCGE Probable lipoate-protein ligase A; TrEMBL-Plants: tr|V7B115|V7B115_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0504900 | 7 | ||
| – | Octanoyltransferase; TAIR: AT1G04640.1 lipoyltransferase 2; Swiss-Prot: sp|Q9SXP7|LIPB_ARATH Octanoyltransferase; TrEMBL-Plants: tr|A0A072VQE6|A0A072VQE6_MEDTR Biotin/lipoate A/B protein ligase family protein; Found in the gene: LotjaGi5g1v0321900 | 14 | ||
| – | Octanoyltransferase; TAIR: AT1G04640.1 lipoyltransferase 2; Swiss-Prot: sp|Q9SXP7|LIPB_ARATH Octanoyltransferase; TrEMBL-Plants: tr|A0A072VQE6|A0A072VQE6_MEDTR Biotin/lipoate A/B protein ligase family protein; Found in the gene: LotjaGi5g1v0321900 | 14 | ||
| – | Octanoyltransferase; TAIR: AT1G04640.1 lipoyltransferase 2; Swiss-Prot: sp|Q9SXP7|LIPB_ARATH Octanoyltransferase; TrEMBL-Plants: tr|A0A072VQE6|A0A072VQE6_MEDTR Biotin/lipoate A/B protein ligase family protein; Found in the gene: LotjaGi5g1v0321900 | 14 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd16444 | CDD | 1 | 7.69 |