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IPR004429 is a Isopropylmalate dehydrogenase.
<p>Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologues. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115.</p> <p>3-isopropylmalate dehydrogenase (IPMDH or LeuB) is the third enzyme in leucine biosynthesis. It catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate [[cite:PUB00032144]]. In the yeast<i>Glarea lozoyensis</i>, 3-isopropylmalate dehydrogenase gloI (EC 1.1.1.85) is required for the biosynthesis of the mycotoxin pneumocandin, a lipohexapeptide of the echinocandin family [[cite:PUB00089640]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | 3-isopropylmalate dehydrogenase activity | Catalysis of the reaction: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD+ = 3-carboxy-4-methyl-2-oxopentanoate + NADH + H+. | ||
| Biological process | Leucine biosynthetic process | The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid. | ||
| Biological process | Oxidation-reduction process | A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: 3-isopropylmalate dehydrogenase, chloroplastic-like [Cicer arietinum] gi|502080646|ref|XP_004486631.1| | 13 | ||
| – | 3-isopropylmalate dehydrogenase; TAIR: AT1G80560.1 isopropylmalate dehydrogenase 2; Swiss-Prot: sp|P29102|LEU3_BRANA 3-isopropylmalate dehydrogenase, chloroplastic; TrEMBL-Plants: tr|A0A151SPV9|A0A151SPV9_CAJCA 3-isopropylmalate dehydrogenase; Found in the gene: LotjaGi3g1v0006000 | 12 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| TIGR00169 | TIGRFAM | 1 | 50.00 |