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IPR004575 is a Cdk-activating kinase assembly factor MAT1/Tfb3.
<p>MAT1/Tfb3 acts as component of the general transcription and DNA repair factor IIH (TFIIH), which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA [[cite:PUB00062852]]. It is a RING finger protein with a characteristic C3HC4 motif located in the N-terminal domain. MAT1/Tfb3 stabilises the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex which then goes on to activate many of the CDK enzymes intimately involved in the cell cycle [[cite:PUB00007681]]. CDK7 forms a stable complex with cyclin H and MAT1/Tfb3 in vivo only when phosphorylated on either one of two residues (Ser164 or Thr170) in its T-loop. The requirement for MAT1/Tfb3 for the activation of CAK can be by-passed by the phosphorylation of CDK7 on the T-loop. The two mechanisms for CDK7 complex stabilisation and activation (MAT1 addition and T-loop phosphorylation), which can operate independently in vitro, actually cooperate under physiological conditions to maintain complex integrity. With prolonged exposure to elevated temperature, dissociation to monomeric subunits occurs in vivo when CDK7 is dephosphorylated, even in the presence of MAT1/Tfb3 [[cite:PUB00007682]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Cellular component | Transcription factor TFIIH holo complex | A complex that is capable of kinase activity directed towards the C-terminal Domain (CTD) of the largest subunit of RNA polymerase II and is essential for initiation at RNA polymerase II promoters in vitro. It is composed of the core TFIIH complex and the TFIIK complex. | ||
| Biological process | Nucleotide-excision repair | A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts). | ||
| Biological process | Positive regulation of cyclin-dependent protein serine/threonine kinase activity | Any process that activates or increases the frequency, rate or extent of CDK activity. | ||
| Molecular function | Cyclin-dependent protein serine/threonine kinase activator activity | Binds to and increases the activity of a cyclin-dependent protein serine/threonine kinase. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic-like [Glycine max] gi|356508382|ref|XP_003522936.1| | 10 | ||
| – | CDK-activating kinase assembly factor MAT1 [Medicago truncatula] gi|357495969|ref|XP_003618273.1| | 4 | ||
| – | CDK-activating kinase assembly factor MAT1 [Medicago truncatula] gi|357495969|ref|XP_003618273.1| | 4 | ||
| – | CDK-activating kinase assembly factor MAT1 [Medicago truncatula] gi|357495969|ref|XP_003618273.1| | 4 | ||
| – | CDK-activating kinase assembly factor; TAIR: AT4G30820.11 cyclin-dependent kinase-activating kinase assembly factor-related / CDK-activating kinase assembly factor-like protein; Swiss-Prot: sp|P51950|MAT1_MARGL CDK-activating kinase assembly factor MAT1; TrEMBL-Plants: tr|G7KI90|G7KI90_MEDTR CDK-activating kinase assembly factor MAT1, putative; Found in the gene: LotjaGi2g1v0254400_LC | 5 | ||
| – | CDK-activating kinase assembly factor; TAIR: AT4G30820.1 cyclin-dependent kinase-activating kinase assembly factor-related / CDK-activating kinase assembly factor-like protein; Swiss-Prot: sp|P51949|MAT1_MOUSE CDK-activating kinase assembly factor MAT1; TrEMBL-Plants: tr|I3S4Z7|I3S4Z7_MEDTR Uncharacterized protein; Found in the gene: LotjaGi2g1v0254400_LC | 5 | ||
| – | CDK-activating kinase assembly factor; TAIR: AT4G30820.1 cyclin-dependent kinase-activating kinase assembly factor-related / CDK-activating kinase assembly factor-like protein; Swiss-Prot: sp|P51950|MAT1_MARGL CDK-activating kinase assembly factor MAT1; TrEMBL-Plants: tr|I3S4Z7|I3S4Z7_MEDTR Uncharacterized protein; Found in the gene: LotjaGi2g1v0254400_LC | 5 | ||
| – | CDK-activating kinase assembly factor; TAIR: AT4G30820.1 cyclin-dependent kinase-activating kinase assembly factor-related / CDK-activating kinase assembly factor-like protein; Swiss-Prot: sp|P51950|MAT1_MARGL CDK-activating kinase assembly factor MAT1; TrEMBL-Plants: tr|I3S4Z7|I3S4Z7_MEDTR Uncharacterized protein; Found in the gene: LotjaGi2g1v0254400_LC | 9 | ||
| – | CDK-activating kinase assembly factor; TAIR: AT4G30820.1 cyclin-dependent kinase-activating kinase assembly factor-related / CDK-activating kinase assembly factor-like protein; Swiss-Prot: sp|P51950|MAT1_MARGL CDK-activating kinase assembly factor MAT1; TrEMBL-Plants: tr|I3S4Z7|I3S4Z7_MEDTR Uncharacterized protein; Found in the gene: LotjaGi2g1v0254400_LC | 9 | ||
| – | CDK-activating kinase assembly factor; TAIR: AT4G30820.1 cyclin-dependent kinase-activating kinase assembly factor-related / CDK-activating kinase assembly factor-like protein; Swiss-Prot: sp|P51949|MAT1_MOUSE CDK-activating kinase assembly factor MAT1; TrEMBL-Plants: tr|C6SW25|C6SW25_SOYBN Putative uncharacterized protein; Found in the gene: LotjaGi2g1v0271400 | 5 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| PTHR12683:SF13 | PANTHER | 1 | 10.00 |