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IPR004810 is a Formyltetrahydrofolate deformylase.
<p>An Escherichia coli gene designated purU has been identified and characterised. The gene codes for a 280-amino-acid protein, PurU ([swissprot:P37051], [ec:3.5.1.10]). PurU is an enzyme that catalyses the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to FH4 and formate [[cite:PUB00007025], [cite:PUB00007026]].</p> <p><reaction>10-formyltetrahydrofolate + H(2)O = formate +tetrahydrofolate</reaction></p> <p>Formyl-FH4 hydrolase generates the formate that is used by purT-encoded 5'-phosphoribosylglycinamide transformylase for step three of de novo purine nucleotide synthesis. Formyl-FH4 hydrolase, a hexamer of 32kDa subunits, is activated by methionine and inhibited by glycine. Heterotropic cooperativity is observed for activation by methionine in the presence of glycine and for inhibition by glycine in the presence of methionine. These results suggest that formyl-FH4 hydrolase is a regulatory enzyme whose main function is to balance the pools of FH4 and C1-FH4 in response to changing growth conditions. The enzyme uses methionine and glycine to sense the pools of C1-FH4 and FH4, respectively.</p> <p>This entry also includes PurU from Arabidopsis, which is involved in photorespiration. It prevents the excessive accumulation of 5-formyl tetrahydrofolate (THF), a potent inhibitor of the Gly decarboxylase/Ser hydroxymethyltransferase complex [[cite:PUB00099957]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | 'de novo' IMP biosynthetic process | The chemical reactions and pathways resulting in the formation of IMP, inosine monophosphate, by the stepwise assembly of a purine ring on ribose 5-phosphate. | ||
| Molecular function | Formyltetrahydrofolate deformylase activity | Catalysis of the reaction: 10-formyltetrahydrofolate + H(2)O = (6S)-5,6,7,8-tetrahydrofolate + formate + H(+). |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Formyltetrahydrofolate deformylase [Medicago truncatula] gi|357502477|ref|XP_003621527.1| | 11 | ||
| – | PREDICTED: formyltetrahydrofolate deformylase-like [Glycine max] gi|356563338|ref|XP_003549921.1| | 15 | ||
| – | Methionyl-tRNA formyltransferase; TAIR: AT5G47435.1 formyltetrahydrofolate deformylase; Swiss-Prot: sp|Q93YQ3|PURU1_ARATH Formyltetrahydrofolate deformylase 1, mitochondrial; TrEMBL-Plants: tr|A0A0L9TQX6|A0A0L9TQX6_PHAAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0202000 | 17 | ||
| – | Formyltetrahydrofolate deformylase; TAIR: AT5G47435.1 formyltetrahydrofolate deformylase; Swiss-Prot: sp|Q93YQ3|PURU1_ARATH Formyltetrahydrofolate deformylase 1, mitochondrial; TrEMBL-Plants: tr|K7KN51|K7KN51_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0202000 | 15 | ||
| – | Methionyl-tRNA formyltransferase; TAIR: AT5G47435.1 formyltetrahydrofolate deformylase; Swiss-Prot: sp|Q93YQ3|PURU1_ARATH Formyltetrahydrofolate deformylase 1, mitochondrial; TrEMBL-Plants: tr|A0A0L9TQX6|A0A0L9TQX6_PHAAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0202000 | 15 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd04875 | CDD | 1 | 20.00 |