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IPR005027 is a Glycosyl transferase, family 43.
<p>The biosynthesis of disaccharides, oligosaccharides and polysaccharides involves the action of hundreds of different glycosyltransferases. These enzymes catalyse the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. A classification of glycosyltransferases using nucleotide diphospho-sugar, nucleotide monophospho-sugar and sugar phosphates ([ec:2.4.1.-]) and related proteins into distinct sequence based families has been described [[cite:PUB00009409]]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. The same three-dimensional fold is expected to occur within each of the families. Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence similarities may have similar 3-D structures and therefore form 'clans'.</p> <p>Glycosyltransferase family 43 [cazy:GT43] comprises enzymes with only one known activity: beta-glucuronyltransferase(GlcAT-I; [ec:2.4.1.135]) [[cite:PUB00081673]].</p> <p>GlcAT-I is a key enzyme involved in the initial steps of proteoglycan synthesis [[cite:PUB00081674]]. GlcAT-I catalyzes the transfer of a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region of trisaccharide Gal-beta-(1-3)-Gal-beta-(1-4)-Xyl of proteoglycans. The enzyme has two subdomains that bind the donor and acceptor substrate separately [[cite:PUB00080741]]. The active site is located at the cleft between both subdomains in which the trisaccharide molecule is oriented perpendicular to the UDP [[cite:PUB00080744]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity | Catalysis of the reaction: UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein. | ||
| Cellular component | Membrane | A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Beta-1,3-glucuronosyltransferase [Lotus japonicus] gi|63087716|emb|CAI93173.1| | 11 | ||
| – | PREDICTED: probable beta-1,4-xylosyltransferase IRX9H-like [Glycine max] gi|356515593|ref|XP_003526483.1| | 7 | ||
| – | Beta-1,3-glucuronosyltransferase [Lotus japonicus] gi|63087716|emb|CAI93173.1| | 12 | ||
| – | Beta-1,3-glucuronosyltransferase [Lotus japonicus] gi|63087716|emb|CAI93173.1| | 12 | ||
| – | PREDICTED: probable beta-1,4-xylosyltransferase IRX14H-like [Glycine max] gi|356563588|ref|XP_003550043.1| | 12 | ||
| – | PREDICTED: probable beta-1,4-xylosyltransferase IRX9-like [Glycine max] gi|356570736|ref|XP_003553541.1| | 14 | ||
| – | PREDICTED: probable beta-1,4-xylosyltransferase IRX14-like [Cicer arietinum] gi|502160779|ref|XP_004511893.1| | 9 | ||
| – | Beta-1,3-glucuronosyltransferase [Lotus japonicus] gi|63087716|emb|CAI93173.1| | 7 | ||
| – | Glycosyltransferases; TAIR: AT1G27600.1 Nucleotide-diphospho-sugar transferases superfamily protein; Swiss-Prot: sp|Q9SXC4|IRX9H_ARATH Probable beta-1,4-xylosyltransferase IRX9H; TrEMBL-Plants: tr|Q50HW5|Q50HW5_LOTJA Glycosyltransferases; Found in the gene: LotjaGi1g1v0063800_LC | 7 | ||
| – | Glycosyltransferases; TAIR: AT1G27600.1 Nucleotide-diphospho-sugar transferases superfamily protein; Swiss-Prot: sp|Q5QM25|GT12_ORYSJ Probable glucuronosyltransferase Os01g0675500; TrEMBL-Plants: tr|I1JUU0|I1JUU0_SOYBN Glycosyltransferases; Found in the gene: LotjaGi1g1v0401800_LC | 7 | ||
| – | Glycosyltransferases; TAIR: AT1G27600.1 Nucleotide-diphospho-sugar transferases superfamily protein; Swiss-Prot: sp|Q9SXC4|IRX9H_ARATH Probable beta-1,4-xylosyltransferase IRX9H; TrEMBL-Plants: tr|Q50HW5|Q50HW5_LOTJA Glycosyltransferases; Found in the gene: LotjaGi1g1v0413300 | 12 | ||
| – | Glycosyltransferases; TAIR: AT2G37090.1 Nucleotide-diphospho-sugar transferases superfamily protein; Swiss-Prot: sp|Q9ZQC6|IRX9_ARATH Probable beta-1,4-xylosyltransferase IRX9; TrEMBL-Plants: tr|I1NA65|I1NA65_SOYBN Glycosyltransferases; Found in the gene: LotjaGi1g1v0693400 | 14 | ||
| – | Glycosyltransferases; TAIR: AT5G67230.1 Nucleotide-diphospho-sugar transferases superfamily protein; Swiss-Prot: sp|Q9FH90|IX14H_ARATH Probable beta-1,4-xylosyltransferase IRX14H; TrEMBL-Plants: tr|A0A0L9UQJ5|A0A0L9UQJ5_PHAAN Glycosyltransferases; Found in the gene: LotjaGi2g1v0404400 | 12 | ||
| – | Glycosyltransferases; TAIR: AT5G67230.1 Nucleotide-diphospho-sugar transferases superfamily protein; Swiss-Prot: sp|Q9FH90|IX14H_ARATH Probable beta-1,4-xylosyltransferase IRX14H; TrEMBL-Plants: tr|A0A1J7GER9|A0A1J7GER9_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0101200 | 12 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 7.14 |