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IPR006194 is a Glycine-tRNA synthetase, heterodimeric.
<p>The aminoacyl-tRNA synthetases (also known as aminoacyl-tRNA ligases) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction [[cite:PUB00079872], [cite:PUB00079873]]. These proteins differ widely in size and oligomeric state, and have limited sequence homology [[cite:PUB00007191]]. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric [[cite:PUB00006477]]. Class II aminoacyl-tRNA synthetases share an anti-parallel β-sheet fold flanked by α-helices [[cite:PUB00000386]], and are mostly dimeric or multimeric, containing at least three conserved regions [[cite:PUB00000723], [cite:PUB00005365], [cite:PUB00004391]]. However, tRNA binding involves an α-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan, valine, and some lysine synthetases (non-eukaryotic group) belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, phenylalanine, proline, serine, threonine, and some lysine synthetases (non-archaeal group), belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c [[cite:PUB00007363]].</p> <p>Glycyl-tRNA synthetase exhibits different oligomeric structures in different organisms (alpha2 beta2 and alpha2) [[cite:PUB00060645]]. This entry represents the alpha and beta subunits of heterodimeric glycine-tRNA synthetases.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Nucleotide binding | Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. | ||
| Molecular function | Glycine-tRNA ligase activity | Catalysis of the reaction: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). | ||
| Molecular function | ATP binding | Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. | ||
| Cellular component | Cytoplasm | All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. | ||
| Biological process | Glycyl-tRNA aminoacylation | The process of coupling glycine to glycyl-tRNA, catalyzed by glycyl-tRNA synthetase. The glycyll-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of a glycine-accepting tRNA. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: glycine--tRNA ligase 2, chloroplastic/mitochondrial-like isoform X2 [Cicer arietinum] gi|502173505|ref|XP_004515345.1| | 8 | ||
| – | PREDICTED: glycine--tRNA ligase 2, chloroplastic/mitochondrial-like isoform X2 [Cicer arietinum] gi|502173505|ref|XP_004515345.1| | 11 | ||
| – | PREDICTED: glycine--tRNA ligase 2, chloroplastic/mitochondrial-like [Fragaria vesca subsp]. vesca] gi|470142584|ref|XP_004306984.1| | 16 | ||
| – | PREDICTED: glycine--tRNA ligase 2, chloroplastic/mitochondrial-like isoform X2 [Cicer arietinum] gi|502173505|ref|XP_004515345.1| | 9 | ||
| – | PREDICTED: glycyl-tRNA synthetase 2, chloroplastic/mitochondrial-like [Glycine max] gi|356503250|ref|XP_003520424.1| | 9 | ||
| – | PREDICTED: glycyl-tRNA synthetase 2, chloroplastic/mitochondrial-like [Glycine max] gi|356503250|ref|XP_003520424.1| | 6 | ||
| – | Glycine-tRNA ligase, alpha subunit; TAIR: AT3G48110.1 glycine-tRNA ligase; Swiss-Prot: sp|Q8L785|SYGM2_ARATH Glycine--tRNA ligase, chloroplastic/mitochondrial 2; TrEMBL-Plants: tr|K7KEC9|K7KEC9_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0067600 | 24 | ||
| – | Glycine-tRNA ligase; TAIR: AT3G48110.1 glycine-tRNA ligase; Swiss-Prot: sp|Q8L785|SYGM2_ARATH Glycine--tRNA ligase, chloroplastic/mitochondrial 2; TrEMBL-Plants: tr|K7KEC9|K7KEC9_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0067600 | 15 | ||
| – | Glycine-tRNA ligase, beta subunit; TAIR: AT3G48110.1 glycine-tRNA ligase; Swiss-Prot: sp|Q8L785|SYGM2_ARATH Glycine--tRNA ligase, chloroplastic/mitochondrial 2; TrEMBL-Plants: tr|K7KEC9|K7KEC9_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0067600 | 14 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| TIGR00211 | TIGRFAM | 1 | 11.11 |