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IPR006357 is a HAD-superfamily hydrolase, subfamily IIA.
<p>These sequences form one of the structural subclasses of the Haloacid Dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases. The superfamily is defined by the presence of three short catalytic motifs [[cite:PUB00003337]]. The classes are defined [[cite:PUB00009589]] based on the location and the observed or predicted fold of a so-called "capping domain" [[cite:PUB00009540]], or the absence of such a domain. Class I consists of sequences in which the capping domain is found in between the first and second catalytic motifs. Class II consists of sequences in which the capping domain is found between the second and third motifs. Class III sequences have no capping domain in either of these positions. The Class IIA capping domain is predicted to consist of a mixed α-β fold with the basic pattern: Helix-Helix-Helix-Sheet-Helix-Loop-Sheet-Helix-Sheet-Helix. Presently, this subfamily covers the eukaryotic phosphoglycolate phosphatase, as well as four further subfamilies covering closely related sequences in eukaryotes, in Gram-positive bacteria and in Gram-negative bacteria. The Escherichia coli NagD gene and the Bacillus subtilis AraL gene are members of this subfamily but are not members of the any of the presently defined equivalogs within it. NagD is part of the NAG operon responsible for N-acetylglucosamine metabolism [[cite:PUB00008243]]. Genes from several organisms have been annotated as NagD, or NagD-like. However, without data on the presence of other members of this pathway, (such as in the case of Yersinia pestis) these assignments should not be given great weight. The AraL gene is similar and is part of the L-arabinose operon [[cite:PUB00008241]]. A gene from<i>Halobacterium</i>has been annotated as AraL, but no other Ara operon genes have been annotated. Many of the genes in this subfamily have been annotated as "pNPPase" "4-nitrophenyl phosphatase" or "NPPase". These all refer to the same activity versus a common lab test compound used to determine phosphatase activity. There is no evidence that this activity is physiologically relevant. Dihydroxyacetone phosphatase from<i>Corynebacterium glutamicum</i>is also a member of this superfamily, and catalyses the dephosphorylation of dihydroxyacetone phosphate to produce 1,3-dihydroxyacetone [[cite:PUB00084382]].</p> <p>The structure of NagD from Escherichia coli (strain K12) has been reported and its activity against various substrates determined. It has high specificity for nucleotide monophosphates, and in particular UMP and GMP. In the context of its occurrence in the NAG operon, it may well be involved in the recycling of cell wall metabolites [[cite:PUB00034756]].</p>
This description is obtained from EB-eye REST.
Transcript | Name | Description | Predicted domains | Domain count |
---|---|---|---|---|
– | Cat eye syndrome critical region protein [Medicago truncatula] gi|357453997|ref|XP_003597279.1| | 10 | ||
– | Cat eye syndrome critical region protein [Medicago truncatula] gi|357453997|ref|XP_003597279.1| | 11 | ||
– | PREDICTED: phosphoglycolate phosphatase-like [Cicer arietinum] gi|502105712|ref|XP_004492869.1| | 14 | ||
– | PREDICTED: 4-nitrophenylphosphatase [Glycine max] gi|356532849|ref|XP_003534982.1| | 15 | ||
– | Phosphoglycolate phosphatase-like protein; TAIR: AT5G36700.1 2-phosphoglycolate phosphatase 1; Swiss-Prot: sp|P0DKC3|PGP1A_ARATH Phosphoglycolate phosphatase 1A, chloroplastic; TrEMBL-Plants: tr|I3T999|I3T999_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0488200 | 15 | ||
– | Phosphoglycolate phosphatase-like protein; TAIR: AT5G36700.1 2-phosphoglycolate phosphatase 1; Swiss-Prot: sp|P0DKC3|PGP1A_ARATH Phosphoglycolate phosphatase 1A, chloroplastic; TrEMBL-Plants: tr|I3T999|I3T999_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0488200 | 11 | ||
– | Hydrolase family protein/HAD-superfamily protein; TAIR: AT3G45740.1 hydrolase family protein / HAD-superfamily protein; Swiss-Prot: sp|O13899|YF38_SCHPO Uncharacterized CDP-alcohol phosphatidyltransferase class-I family protein C22A12.08c; TrEMBL-Plants: tr|A0A151SV01|A0A151SV01_CAJCA Uncharacterized protein YKR070W family; Found in the gene: LotjaGi3g1v0269400 | 12 | ||
– | HAD-superfamily hydrolase, subfamily IIA; TAIR: AT5G10460.1 Haloacid dehalogenase-like hydrolase (HAD) superfamily protein; Swiss-Prot: sp|O32125|YUTF_BACSU Acid sugar phosphatase; TrEMBL-Plants: tr|G7JNP6|G7JNP6_MEDTR HAD-family hydrolase IIA; Found in the gene: LotjaGi4g1v0067600 | 12 | ||
– | Phosphoglycolate phosphatase; TAIR: AT5G47760.1 2-phosphoglycolate phosphatase 2; Swiss-Prot: sp|Q8GWU0|PGP2_ARATH Phosphoglycolate phosphatase 2; TrEMBL-Plants: tr|K7LBA1|K7LBA1_SOYBN Phosphoglycolate phosphatase; Found in the gene: LotjaGi6g1v0281700_LC | 15 |
A list of co-occurring predicted domains within the L. japonicus gene space:
Predicted domain | Source | Observations | Saturation (%) |
---|---|---|---|
TIGR01460 | TIGRFAM | 1 | 11.11 |