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IPR013471 is a Ribonuclease Z/BN.
<p>This entry includes ribonuclease Z (RNase Z) and its orthologues, such as RNase BN (also known as elaC) from E.coli. They are ribonucleases that have functions in tRNA-3' processing. RNase Z orthologues are present in all eukaryotes and archaea sequenced so far, and are widely distributed in bacteria [[cite:PUB00083320], [cite:PUB00083319]].</p> <p>All tRNAs are synthesized as precursor molecules and their maturation to functional tRNAs requires several processing steps. First, the removal of the 5'-leader is catalysed by the endonucleolytic ribozyme RNase P. Second, the removal of the 3'-trailer sequences, which is either a single-step endonucleolytic process or a two-step process involving downstream cleavage by an endonuclease followed by exonucleolytic trimming to generate the mature 3'-end [[cite:PUB00083319]].</p> <p>The first evidence for the maturation of tRNA 3'-ends by a single endonucleolytic cleavage reaction came from eukaryotes. In these cases, the cleavage of the 3'-end is carried out by RNase Z. In most cases, RNase Z removes the 3'-trailer sequence by cleaving directly after the discriminator nucleotide. Interestingly, the CCA motif present in all mature tRNAs is a general anti-determinant for RNase Z activity. The activity of some RNase Z homologues found in bacterial and archaea have also been shown to be inhibited by the presence of a CCA motif [[cite:PUB00083319]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Endoribonuclease activity, producing 5'-phosphomonoesters | Catalysis of the hydrolysis of ester linkages within ribonucleic acids by creating internal breaks to yield 5'-phosphomonoesters. | ||
| Biological process | TRNA 3'-trailer cleavage | Cleavage of the 3'-end of the pre-tRNA as part of the process of generating the mature 3'-end of the tRNA; may involve endonucleolytic or exonucleolytic cleavage, or both. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | PREDICTED: zinc phosphodiesterase ELAC protein 2-like [Glycine max] gi|356557289|ref|XP_003546950.1| | 8 | ||
| – | Ribonuclease Z; TAIR: AT3G16260.1 tRNAse Z4; Swiss-Prot: sp|Q9BQ52|RNZ2_HUMAN Zinc phosphodiesterase ELAC protein 2; TrEMBL-Plants: tr|I1M5I2|I1M5I2_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0019500 | 11 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| mobidb-lite | MobiDBLite | 1 | 50.00 |