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IPR013528 is a Hydroxymethylglutaryl-coenzyme A synthase, N-terminal.
<p>This entry represents the N-terminal domain of HMG-CoA synthase enzymes from both eukaryotes and prokaryotes.</p> <p>Hydroxymethylglutaryl-CoA synthase ([ec:2.3.3.10]) catalyses the condensation of acetyl-CoA with acetoacetyl-CoA to produce HMG-CoA and CoA, the second reaction in the mevalonate-dependent isoprenoid biosynthesis pathway. HMG-CoA synthase contains an important catalytic cysteine residue that acts as a nucleophile in the first step of the reaction: the acetylation of the enzyme by acetyl-CoA (its first substrate) to produce an acetyl-enzyme thioester, releasing the reduced coenzyme A. The subsequent nucleophilic attack on acetoacetyl-CoA (its second substrate) leads to the formation of HMG-CoA [[cite:PUB00036056]].</p> <p>HMG-CoA synthase occurs in eukaryotes, archaea and certain bacteria [[cite:PUB00036057]]. In vertebrates, there are two isozymes located in different subcellular compartments: a cytosolic form that is the starting point of the mevalonate pathway (leads to cholesterol and other sterolic and isoprenoid compounds), and a mitochondrial form responsible for ketone body biosynthesis. HMG-CoA is also found in other eukaryotes such as insects, plants and fungi [[cite:PUB00036058]]. In bacteria, isoprenoid precursors are generally synthesised via an alternative, non-mevalonate pathway, however a number of Gram-positive pathogens utilise a mevalonate pathway involving HMG-CoA synthase that is parallel to that found in eukaryotes [[cite:PUB00036059], [cite:PUB00036060]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Hydroxymethylglutaryl-CoA synthase activity | Catalysis of the reaction: acetoacetyl-CoA + acetyl-CoA + H(2)O = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+). | ||
| Biological process | Isoprenoid biosynthetic process | The chemical reactions and pathways resulting in the formation of any isoprenoid compound, isoprene (2-methylbuta-1,3-diene) or compounds containing or derived from linked isoprene (3-methyl-2-butenylene) residues. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Hydroxymethylglutaryl-CoA synthase [Medicago truncatula] gi|357500885|ref|XP_003620731.1| | 14 | ||
| – | PREDICTED: hydroxymethylglutaryl-CoA synthase-like [Glycine max] gi|356539911|ref|XP_003538436.1| | 14 | ||
| – | PREDICTED: hydroxymethylglutaryl-CoA synthase-like [Glycine max] gi|356563228|ref|XP_003549866.1| | 14 | ||
| – | Hydroxymethylglutaryl-CoA synthase; TAIR: AT4G11820.2 hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase; Swiss-Prot: sp|P54873|HMCS_ARATH Hydroxymethylglutaryl-CoA synthase; TrEMBL-Plants: tr|G7KQF5|G7KQF5_MEDTR 3-hydroxy-3-methylglutaryl coenzyme A synthase; Found in the gene: LotjaGi2g1v0151200 | 14 | ||
| – | Hydroxymethylglutaryl-CoA synthase; TAIR: AT4G11820.2 hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase; Swiss-Prot: sp|P54873|HMCS_ARATH Hydroxymethylglutaryl-CoA synthase; TrEMBL-Plants: tr|I1LGI7|I1LGI7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0430500 | 14 | ||
| – | Hydroxymethylglutaryl-CoA synthase; TAIR: AT4G11820.2 hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase; Swiss-Prot: sp|P54873|HMCS_ARATH Hydroxymethylglutaryl-CoA synthase; TrEMBL-Plants: tr|I1LGI7|I1LGI7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0430500 | 14 | ||
| – | Hydroxymethylglutaryl-CoA synthase; TAIR: AT4G11820.2 hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase; Swiss-Prot: sp|P54873|HMCS_ARATH Hydroxymethylglutaryl-CoA synthase; TrEMBL-Plants: tr|I1LGI7|I1LGI7_SOYBN Uncharacterized protein; Found in the gene: LotjaGi2g1v0430500 | 14 | ||
| – | Hydroxymethylglutaryl-CoA synthase; TAIR: AT4G11820.2 hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase; Swiss-Prot: sp|P54873|HMCS_ARATH Hydroxymethylglutaryl-CoA synthase; TrEMBL-Plants: tr|A0A075EBV0|A0A075EBV0_ASTME 3-hydroxy-3-methylglutaryl coenzyme A synthase; Found in the gene: LotjaGi4g1v0218500 | 14 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd00827 | CDD | 1 | 12.50 |