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IPR013775 is a Alpha-amylase, plant.
<p>O-Glycosyl hydrolases ([ec:3.2.1.]) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [[cite:PUB00004870], [cite:PUB00005266]]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.</p> <p>Alpha-amylase is classified as family 13 ([cazy:GH13]) of the glycosyl hydrolases and is present in archaea, bacteria, fungi, plants and animals. Alpha-amylase is an essential enzyme in alpha-glucan metabolism, acting to catalyse the hydrolysis of alpha-1,4-glucosidic bonds of glycogen, starch and related polysaccharides. Although all alpha-amylases possess the same catalytic function, they can vary with respect to sequence. In general, they are composed of three domains: a TIM barrel containing the active site residues and chloride ion-binding site (domain A), a long loop region inserted between the third β-strand and the α-helix of domain A that contains calcium-binding site(s) (domain B), and a C-terminal β-sheet domain that appears to show some variability in sequence and length between amylases (domain C) [[cite:PUB00027666]]. Amylases have at least one conserved calcium-binding site, as calcium is essential for the stability of the enzyme. The chloride-binding functions to activate the enzyme, which acts by a two-step mechanism involving a catalytic nucleophile base (usually an Asp) and a catalytic proton donor (usually a Glu) that are responsible for the formation of the beta-linked glycosyl-enzyme intermediate.</p> <p>This entry represents a subfamily of alpha-amylase proteins that are found in plants.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Alpha-amylase activity | Catalysis of the endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more alpha-(1->4)-linked D-glucose units. | ||
| Molecular function | Calcium ion binding | Interacting selectively and non-covalently with calcium ions (Ca2+). | ||
| Biological process | Carbohydrate metabolic process | The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Alpha-amylase [Medicago truncatula] gi|357437821|ref|XP_003589186.1| | 27 | ||
| – | Alpha-amylase; TAIR: AT1G76130.1 alpha-amylase-like 2; Swiss-Prot: sp|Q8LFG1|AMY2_ARATH Probable alpha-amylase 2; TrEMBL-Plants: tr|C6TCX9|C6TCX9_SOYBN Alpha-amylase; Found in the gene: LotjaGi5g1v0002500 | 20 | ||
| – | Alpha-amylase; TAIR: AT1G76130.1 alpha-amylase-like 2; Swiss-Prot: sp|Q8LFG1|AMY2_ARATH Probable alpha-amylase 2; TrEMBL-Plants: tr|C6TCX9|C6TCX9_SOYBN Alpha-amylase; Found in the gene: LotjaGi5g1v0002500 | 20 | ||
| – | Alpha-amylase; TAIR: AT1G76130.1 alpha-amylase-like 2; Swiss-Prot: sp|Q8LFG1|AMY2_ARATH Probable alpha-amylase 2; TrEMBL-Plants: tr|C6TCX9|C6TCX9_SOYBN Alpha-amylase; Found in the gene: LotjaGi5g1v0002500 | 20 | ||
| – | Alpha-amylase; TAIR: AT4G25000.1 alpha-amylase-like protein; Swiss-Prot: sp|P17859|AMYA_VIGMU Alpha-amylase; TrEMBL-Plants: tr|A0A151S840|A0A151S840_CAJCA Alpha-amylase; Found in the gene: LotjaGi5g1v0028300 | 25 | ||
| – | Alpha-amylase; TAIR: AT4G25000.1 alpha-amylase-like protein; Swiss-Prot: sp|P17859|AMYA_VIGMU Alpha-amylase; TrEMBL-Plants: tr|A0A151S7Y0|A0A151S7Y0_CAJCA Alpha-amylase; Found in the gene: LotjaGi5g1v0028400 | 25 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd11314 | CDD | 1 | 16.67 |