Description

IPR018376 is a Enoyl-CoA hydratase/isomerase, conserved site.

<p>The crotonase superfamily is comprised of mechanistically diverse proteins that share a conserved trimeric quaternary structure (sometimes a hexamer consisting of a dimer of trimers), the core of which consists of 4 turns of a (β/β/α)n superhelix. Some enzymes in the superfamily have been shown to display dehalogenase, hydratase, and isomerase activities, while others have been implicated in carbon-carbon bond formation and cleavage as well as the hydrolysis of thioesters [[cite:PUB00035962]]. However, these different enzymes share the need to stabilise an enolate anion intermediate derived from an acyl-CoA substrate. This is accomplished by two structurally conserved peptidic NH groups that provide hydrogen bonds to the carbonyl moieties of the acyl-CoA substrates and form an "oxyanion hole". The CoA thioester derivatives bind in a characteristic hooked shape and a conserved tunnel binds the pantetheine group of CoA, which links the 3'-phosphate ADP binding site to the site of reaction [[cite:PUB00035964]]. Enzymes in the crotonase superfamily include:</p> <ul> <li>Enoyl-CoA hydratase (crotonase; [ec:4.2.1.17]), which catalyses the hydration of 2-trans-enoyl-CoA into 3-hydroxyacyl-CoA [[cite:PUB00027360]].</li> <li>3-2 trans-enoyl-CoA isomerase (or dodecenoyl-CoA isomerise; [ec:5.3.3.8]), which shifts the 3-double bond of the intermediates of unsaturated fatty acid oxidation to the 2-trans position [[cite:PUB00000748]].</li> <li>3-hydroxybutyryl-CoA dehydratase (crotonase; [ec:4.2.1.55]), a bacterial enzyme involved in the butyrate/butanol-producing pathway.</li> <li>4-Chlorobenzoyl-CoA dehalogenase ([ec:3.8.1.6]), a Pseudomonas enzyme which catalyses the conversion of 4-chlorobenzoate-CoA to 4-hydroxybenzoate-CoA [[cite:PUB00035965]].</li> <li>Dienoyl-CoA isomerase, which catalyses the isomerisation of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA [[cite:PUB00024165]].</li> <li>Naphthoate synthase (MenB, or DHNA synthetase; [ec:4.1.3.36]), a bacterial enzyme involved in the biosynthesis of menaquinone (vitamin K2) [[cite:PUB00033366]].</li> <li>Carnitine racemase (gene caiD), which catalyses the reversible conversion of crotonobetaine to L-carnitine in Escherichia coli [[cite:PUB00035966]].</li> <li>Methylmalonyl CoA decarboxylase (MMCD; [ec:7.2.4.3]), which has a hexameric structure (dimer of trimers) [[cite:PUB00024526]].</li> <li>Carboxymethyl Proline synthase (CarB), which is involved in carbapenem biosynthesis [[cite:PUB00035963]].</li> <li>6-oxo camphor hydrolase, which catalyses the desymmetrization of bicyclic beta-diketones to optically active keto acids [[cite:PUB00031259]].</li> <li>The alpha subunit of fatty oxidation complex, a multi-enzyme complex that catalyses the last three reactions in the fatty acid beta-oxidation cycle [[cite:PUB00035967]].</li> <li>AUH protein, a bifunctional RNA-binding homologue of enoyl-CoA hydratase [[cite:PUB00025845]].</li> </ul>

This description is obtained from EB-eye REST.

Associated GO terms

GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on April 27, 2020, while the GO metadata was last updated on April 27, 2020.

Associated Lotus transcripts 9

Co-occuring domains 1

A list of co-occurring predicted domains within the L. japonicus gene space: