Your browser is unable to support new features implemented in HTML5 and CSS3 to render this site as intended. Your experience may suffer from functionality degradation but the site should remain usable. We strongly recommend the latest version of Google Chrome, OS X Safari or Mozilla Firefox. As Safari is bundled with OS X, if you are unable to upgrade to a newer version of OS X, we recommend using an open source browser. Dismiss message
IPR028885 is a Adenylyltransferase and sulfurtransferase MOCS3/Uba4.
<p>This entry includes adenylyltransferase and sulfurtransferase MOCS3 and its homologue, Uba4, from yeasts.</p> <p>In budding yeasts, Uba4 receives sulphur from Nfs1 and Tum1 [[cite:PUB00069706]]. Uba4 then forms an acyl adenylate intermediate with the C-terminal of the Urm1 protein, and then forms an acyl disulfide bond between Uba4 Cys397 and the Urm1 C-terminal glycin [[cite:PUB00069706]]. Subsequently, the activated Urm1 acts as a sulphur carrier in the process of eukaryotic transfer RNA (tRNA) thiolation [[cite:PUB00069594]].</p> <p>MOCS3 plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln) [[cite:PUB00053951]]. It is also essential during biosynthesis of the molybdenum cofactor [[cite:PUB00069588], [cite:PUB00069589]]. MOCS3 acts by mediating the C-terminal thiocarboxylation of sulfur carriers Urm1 and MOCS2A (the small subunit of molybdopterin synthase) [[cite:PUB00069594], [cite:PUB00069587], [cite:PUB00048703], [cite:PUB00069593]].</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Biological process | TRNA wobble position uridine thiolation | The process in which a uridine residue at position 34 in the anticodon of a tRNA is post-transcriptionally thiolated at the C2 position. This process involves transfer of a sulfur from cysteine to position C2 by several steps. | ||
| Molecular function | Thiosulfate sulfurtransferase activity | Catalysis of the reaction: hydrogen cyanide + thiosulfate = H(+) + sulfite + thiocyanate. | ||
| Cellular component | Cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Adenylyltransferase and sulfurtransferase MOCS3; TAIR: AT5G55130.1 co-factor for nitrate, reductase and xanthine dehydrogenase 5; Swiss-Prot: sp|Q9ZNW0|MOCS3_ARATH Adenylyltransferase and sulfurtransferase MOCS3; TrEMBL-Plants: tr|G7K8M6|G7K8M6_MEDTR Adenylyltransferase and sulfurtransferase MOCS3; Found in the gene: LotjaGi1g1v0025600 | 18 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd01526 | CDD | 1 | 100.00 |