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IPR035985 is a Ubiquitin-activating enzyme-like.
<p>This entry describes the ubiquitin-activating enzymes E1, ubiquitin-like modifier-activating enzymes, and related enzymes. It also includes adenylyltransferase and sulfurtransferase from the MOCS3 (humans) and UBA4 (yeast) complexes, which are enzymes involved in ubiquitin-like pathways. These enzymes play critical roles in activating ubiquitin-like proteins (UBLs) such as Urm1 [[cite:PUB00160412], [cite:PUB00160411]].</p> <p>The post-translational attachment of ubiquitin ([interpro:IPR000626]) to proteins (ubiquitinylation) alters the function, location or trafficking of a protein, or targets it to the 26S proteasome for degradation [[cite:PUB00015621], [cite:PUB00015619], [cite:PUB00015625]]. Ubiquitinylation is an ATP-dependent process that involves the action of at least three enzymes: a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2, [interpro:IPR000608]), and a ubiquitin ligase (E3, [interpro:IPR000569], [interpro:IPR003613]), which work sequentially in a cascade [[cite:PUB00015620]]. The E1 enzyme is responsible for activating ubiquitin, the first step in ubiquitinylation. The E1 enzyme hydrolyses ATP and adenylates the C-terminal glycine residue of ubiquitin, and then links this residue to the active site cysteine of E1, yielding a ubiquitin-thioester and free AMP. To be fully active, E1 must non-covalently bind to and adenylate a second ubiquitin molecule. The E1 enzyme can then transfer the thioester-linked ubiquitin molecule to a cysteine residue on the ubiquitin-conjugating enzyme, E2, in an ATP-dependent reaction.</p>
This description is obtained from EB-eye REST.
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| GO term | Namespace | Name | Definition | Relationships |
|---|---|---|---|---|
| Molecular function | Ubiquitin-like modifier activating enzyme activity | Catalysis of the activation of small proteins, such as ubiquitin or ubiquitin-like proteins, through the formation of an ATP-dependent high-energy thiolester bond. |
| Transcript | Name | Description | Predicted domains | Domain count |
|---|---|---|---|---|
| – | Adenylyltransferase and sulfurtransferase MOCS3; TAIR: AT5G55130.1 co-factor for nitrate, reductase and xanthine dehydrogenase 5; Swiss-Prot: sp|Q9ZNW0|MOCS3_ARATH Adenylyltransferase and sulfurtransferase MOCS3; TrEMBL-Plants: tr|G7K8M6|G7K8M6_MEDTR Adenylyltransferase and sulfurtransferase MOCS3; Found in the gene: LotjaGi1g1v0025600 | 18 | ||
| – | Adenylyltransferase and sulfurtransferase MOCS3; TAIR: AT5G55130.1 co-factor for nitrate, reductase and xanthine dehydrogenase 5; Swiss-Prot: sp|Q9ZNW0|MOCS3_ARATH Adenylyltransferase and sulfurtransferase MOCS3; TrEMBL-Plants: tr|I1JJC5|I1JJC5_SOYBN Adenylyltransferase and sulfurtransferase MOCS3; Found in the gene: LotjaGi1g1v0025600 | 14 | ||
| – | Adenylyltransferase and sulfurtransferase MOCS3; TAIR: AT5G55130.1 co-factor for nitrate, reductase and xanthine dehydrogenase 5; Swiss-Prot: sp|Q9ZNW0|MOCS3_ARATH Adenylyltransferase and sulfurtransferase MOCS3; TrEMBL-Plants: tr|I1JJC5|I1JJC5_SOYBN Adenylyltransferase and sulfurtransferase MOCS3; Found in the gene: LotjaGi1g1v0025600 | 14 | ||
| – | Adenylyltransferase and sulfurtransferase MOCS3; TAIR: AT5G37530.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|P36101|TCD2_YEAST tRNA threonylcarbamoyladenosine dehydratase 2; TrEMBL-Plants: tr|K7M8D6|K7M8D6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0095800 | 12 | ||
| – | Adenylyltransferase and sulfurtransferase MOCS3; TAIR: AT5G37530.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|O13861|TCD_SCHPO tRNA threonylcarbamoyladenosine dehydratase; TrEMBL-Plants: tr|K7M8D6|K7M8D6_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0095800 | 12 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|K7KA83|K7KA83_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0116200 | 39 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT5G06460.1 ubiquitin activating enzyme 2; Swiss-Prot: sp|P92974|UBE12_ARATH Ubiquitin-activating enzyme E1 2; TrEMBL-Plants: tr|G7KEA9|G7KEA9_MEDTR Ubiquitin-activating enzyme E1 1; Found in the gene: LotjaGi1g1v0116200 | 39 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|G7KEA8|G7KEA8_MEDTR Ubiquitin-activating enzyme E1 1; Found in the gene: LotjaGi1g1v0116200 | 38 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|G7KEA8|G7KEA8_MEDTR Ubiquitin-activating enzyme E1 1; Found in the gene: LotjaGi1g1v0116200 | 38 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|G7KEA8|G7KEA8_MEDTR Ubiquitin-activating enzyme E1 1; Found in the gene: LotjaGi1g1v0116200 | 38 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|G7KEA9|G7KEA9_MEDTR Ubiquitin-activating enzyme E1 1; Found in the gene: LotjaGi1g1v0116200 | 39 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G21470.1 SUMO-activating enzyme 2; Swiss-Prot: sp|Q9SJT1|SAE2_ARATH SUMO-activating enzyme subunit 2; TrEMBL-Plants: tr|V7ALR1|V7ALR1_PHAVU Uncharacterized protein; Found in the gene: LotjaGi1g1v0275000_LC | 8 | ||
| – | Ubiquitin-like modifier-activating enzyme 5; TAIR: AT1G05350.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|O23034|UBA5_ARATH Ubiquitin-like modifier-activating enzyme 5; TrEMBL-Plants: tr|I3S0U0|I3S0U0_LOTJA Uncharacterized protein; Found in the gene: LotjaGi1g1v0338800 | 11 | ||
| – | SUMO-activating enzyme 2; TAIR: AT2G21470.1 SUMO-activating enzyme 2; Swiss-Prot: sp|Q9SJT1|SAE2_ARATH SUMO-activating enzyme subunit 2; TrEMBL-Plants: tr|I1LVC3|I1LVC3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0061900 | 17 | ||
| – | SUMO-activating enzyme 2; TAIR: AT2G21470.1 SUMO-activating enzyme 2; Swiss-Prot: sp|Q9SJT1|SAE2_ARATH SUMO-activating enzyme subunit 2; TrEMBL-Plants: tr|I1LVC3|I1LVC3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi3g1v0061900 | 16 | ||
| – | Adenylyltransferase and sulfurtransferase; TAIR: AT2G21470.1 SUMO-activating enzyme 2; Swiss-Prot: sp|Q9SJT1|SAE2_ARATH SUMO-activating enzyme subunit 2; TrEMBL-Plants: tr|V7ALR1|V7ALR1_PHAVU Uncharacterized protein; Found in the gene: LotjaGi3g1v0323700_LC | 8 | ||
| – | NEDD8-activating enzyme E1 regulatory subunit; TAIR: AT1G05180.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|P42744|AXR1_ARATH NEDD8-activating enzyme E1 regulatory subunit AXR1; TrEMBL-Plants: tr|I3SW23|I3SW23_LOTJA NEDD8-activating enzyme E1 regulatory subunit; Found in the gene: LotjaGi3g1v0466500 | 10 | ||
| – | NEDD8-activating enzyme E1 regulatory subunit; TAIR: AT1G05180.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|P42744|AXR1_ARATH NEDD8-activating enzyme E1 regulatory subunit AXR1; TrEMBL-Plants: tr|I3SW23|I3SW23_LOTJA NEDD8-activating enzyme E1 regulatory subunit; Found in the gene: LotjaGi3g1v0466500 | 10 | ||
| – | NEDD8-activating enzyme E1 regulatory subunit; TAIR: AT1G05180.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|P42744|AXR1_ARATH NEDD8-activating enzyme E1 regulatory subunit AXR1; TrEMBL-Plants: tr|I3SW23|I3SW23_LOTJA NEDD8-activating enzyme E1 regulatory subunit; Found in the gene: LotjaGi3g1v0466500 | 5 | ||
| – | NEDD8-activating enzyme E1 regulatory subunit; TAIR: AT1G05180.1 NAD(P)-binding Rossmann-fold superfamily protein; Swiss-Prot: sp|P42744|AXR1_ARATH NEDD8-activating enzyme E1 regulatory subunit AXR1; TrEMBL-Plants: tr|I3SW23|I3SW23_LOTJA NEDD8-activating enzyme E1 regulatory subunit; Found in the gene: LotjaGi3g1v0466500 | 5 | ||
| – | Ubiquitin-like modifier-activating enzyme atg7; TAIR: AT5G45900.1 ThiF family protein; Swiss-Prot: sp|Q94CD5|ATG7_ARATH Ubiquitin-like modifier-activating enzyme atg7; TrEMBL-Plants: tr|A0A0B2RJ97|A0A0B2RJ97_GLYSO Ubiquitin-like modifier-activating enzyme atg7; Found in the gene: LotjaGi3g1v0547800 | 16 | ||
| – | Ubiquitin-like modifier-activating enzyme atg7; TAIR: AT5G45900.1 ThiF family protein; Swiss-Prot: sp|Q94CD5|ATG7_ARATH Ubiquitin-like modifier-activating enzyme atg7; TrEMBL-Plants: tr|A0A0B2RJ97|A0A0B2RJ97_GLYSO Ubiquitin-like modifier-activating enzyme atg7; Found in the gene: LotjaGi3g1v0547800 | 16 | ||
| – | Ubiquitin-like modifier-activating enzyme atg7; TAIR: AT5G45900.1 ThiF family protein; Swiss-Prot: sp|Q94CD5|ATG7_ARATH Ubiquitin-like modifier-activating enzyme atg7; TrEMBL-Plants: tr|A0A0B2RJ97|A0A0B2RJ97_GLYSO Ubiquitin-like modifier-activating enzyme atg7; Found in the gene: LotjaGi3g1v0547800 | 16 | ||
| – | SUMO-activating enzyme subunit 1; TAIR: AT4G24940.1 SUMO-activating enzyme 1A; Swiss-Prot: sp|Q8VY78|SAE1A_ARATH SUMO-activating enzyme subunit 1A; TrEMBL-Plants: tr|A0A1J7HZ69|A0A1J7HZ69_LUPAN Uncharacterized protein; Found in the gene: LotjaGi4g1v0069200 | 8 | ||
| – | NEDD8-activating enzyme E1 catalytic subunit; TAIR: AT5G19180.1 E1 C-terminal related 1; Swiss-Prot: sp|O65041|UBA3_ARATH NEDD8-activating enzyme E1 catalytic subunit; TrEMBL-Plants: tr|I1KNA2|I1KNA2_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0352900 | 17 | ||
| – | SUMO-activating enzyme subunit 1; TAIR: AT4G24940.1 SUMO-activating enzyme 1A; Swiss-Prot: sp|Q8VY78|SAE1A_ARATH SUMO-activating enzyme subunit 1A; TrEMBL-Plants: tr|I3SJS2|I3SJS2_LOTJA Uncharacterized protein; Found in the gene: LotjaGi4g1v0418200 | 7 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|A0A0L9VCA7|A0A0L9VCA7_PHAAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0126000 | 36 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|I1MZW3|I1MZW3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0126000 | 35 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|A0A0L9VCA7|A0A0L9VCA7_PHAAN Uncharacterized protein; Found in the gene: LotjaGi6g1v0126000 | 36 | ||
| – | Ubiquitin activating enzyme E1; TAIR: AT2G30110.1 ubiquitin-activating enzyme 1; Swiss-Prot: sp|P93028|UBE11_ARATH Ubiquitin-activating enzyme E1 1; TrEMBL-Plants: tr|I1MZW3|I1MZW3_SOYBN Uncharacterized protein; Found in the gene: LotjaGi6g1v0126000 | 35 |
A list of co-occurring predicted domains within the L. japonicus gene space:
| Predicted domain | Source | Observations | Saturation (%) |
|---|---|---|---|
| cd01491 | CDD | 1 | 3.33 |