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| Field | Value |
|---|---|
| Namespace | Molecular function |
| Short description | TRNA dihydrouridine synthase activity |
| Full defintion | Catalysis of the reaction: tRNA-uracil + acceptor = tRNA-dihydrouridine + reduced acceptor. |
| Subterm of |
The relationship of GO:0017150 with other GO terms.
| Relationship type | GO terms |
|---|---|
| Is a | |
| Regulates | n.a. |
| Part of | n.a. |
| Positively regulates | n.a. |
| Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0017150, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
| Field | Value |
|---|---|
| PMID | Identification of the tRNA-dihydrouridine synthase family. J Biol Chem. 2002 Jul 12; 277 (28): 25090–5.PMID: 11983710 5,6-Dihydrouridine (D) is a modified base found abundantly in the D-loops of tRNA from Archaea, Bacteria, and Eukarya. D is thought to be formed post-transcriptionally by the reduction of uridines in tRNA transcripts. Despite its abundance, no enzymes that catalyze D-formation have been identified. Using comparative genomics and computational methods we have identified members of the cluster of orthologous genes, COG0042, as putative dihydrouridine synthase encoding genes. Escherichia coli contains three COG0042 family members (yjbN, yhdG, and yohI). Strains were created where one, two, or all three of the COG0042 genes were deleted. Purified tRNA samples were investigated from the three single and the three double knockout strains, as well as from the triple deletion strain. The results showed that the COG0042 gene family is responsible for tRNA-dihydrouridine synthase activity in E. coli. They also suggest that the COG0042-encoded family members act site-specifically on the tRNA D-loop and contain non-redundant catalytic functions in vivo. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
| Transcript | Name | Description | GO terms | GO count |
|---|---|---|---|---|
| – | PREDICTED: tRNA-dihydrouridine(20) synthase [NAD](P)+] gi|502154498|ref|XP_004509720.1| | 4 | ||
| – | PREDICTED: tRNA-dihydrouridine synthase A-like isoform X3 [Cicer arietinum] gi|502127969|ref|XP_004499894.1| | 4 | ||
| – | PREDICTED: tRNA-dihydrouridine synthase A-like [Glycine max] gi|356537294|ref|XP_003537164.1| | 4 | ||
| – | PREDICTED: tRNA-dihydrouridine synthase A-like [Glycine max] gi|356537294|ref|XP_003537164.1| | 4 | ||
| – | PREDICTED: tRNA-dihydrouridine synthase 3-like [Glycine max] gi|356508136|ref|XP_003522816.1| | 4 | ||
| – | PREDICTED: tRNA-dihydrouridine synthase 3-like [Glycine max] gi|356508136|ref|XP_003522816.1| | 4 | ||
| – | PREDICTED: tRNA-dihydrouridine synthase A-like isoform X2 [Cicer arietinum] gi|502146150|ref|XP_004506326.1| | 4 | ||
| – | 4 | |||
| – | PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD](P)(+)] gi|502152821|ref|XP_004509110.1| | 4 | ||
| – | TRNA-dihydrouridine(47) synthase [NAD](P)(+)]; TAIR: AT4G38890.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q9T0J6|DUS3L_ARATH tRNA-dihydrouridine(47) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|K7KHG3|K7KHG3_SOYBN tRNA-dihydrouridine(47) synthase [NAD](P)(+)]; Found in the gene: LotjaGi1g1v0562300 | 4 | ||
| – | TRNA-dihydrouridine(47) synthase [NAD](P)(+)]; TAIR: AT4G38890.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q9T0J6|DUS3L_ARATH tRNA-dihydrouridine(47) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|K7KHG3|K7KHG3_SOYBN tRNA-dihydrouridine(47) synthase [NAD](P)(+)]; Found in the gene: LotjaGi1g1v0562300 | 4 | ||
| – | TRNA-dihydrouridine synthase; TAIR: AT5G47970.1 Aldolase-type TIM barrel family protein; Swiss-Prot: sp|Q9KUX9|DUSA_VIBCH tRNA-dihydrouridine(20/20a) synthase; TrEMBL-Plants: tr|K7LI80|K7LI80_SOYBN tRNA-dihydrouridine synthase; Found in the gene: LotjaGi3g1v0121800 | 5 | ||
| – | Trna-dihydrouridine(47) synthase [nad](P)(+)]-like protein; TAIR: AT3G49640.1 Aldolase-type TIM barrel family protein; Swiss-Prot: sp|Q9NX74|DUS2L_HUMAN tRNA-dihydrouridine(20) synthase [NAD](P)+]-like; TrEMBL-Plants: tr|A0A151SZ46|A0A151SZ46_CAJCA tRNA-dihydrouridine synthase; Found in the gene: LotjaGi3g1v0179300 | 4 | ||
| – | TRNA-dihydrouridine synthase; TAIR: AT3G63510.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q8Z1T1|DUSA_SALTI tRNA-dihydrouridine(20/20a) synthase; TrEMBL-Plants: tr|I3SNH2|I3SNH2_LOTJA Uncharacterized protein; Found in the gene: LotjaGi3g1v0550700 | 5 | ||
| – | TRNA-dihydrouridine synthase-like protein; TAIR: AT5G67220.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q8C2P3|DUS1L_MOUSE tRNA-dihydrouridine(16/17) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|I1K265|I1K265_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0101600 | 4 | ||
| – | TRNA-dihydrouridine synthase-like protein; TAIR: AT5G67220.1 FMN-linked oxidoreductases superfamily protein; Swiss-Prot: sp|Q8C2P3|DUS1L_MOUSE tRNA-dihydrouridine(16/17) synthase [NAD](P)(+)]-like; TrEMBL-Plants: tr|I1K265|I1K265_SOYBN Uncharacterized protein; Found in the gene: LotjaGi4g1v0101600 | 4 |
A list of co-occurring GO terms within the L. japonicus gene space:
| GO term | Namespace | Name | Observations | Saturation (%) |
|---|---|---|---|---|
| Biological process | Oxidation-reduction process | 1 | 6.25 |