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Field | Value |
---|---|
Namespace | Biological process |
Short description | Peptidyl-diphthamide biosynthetic process from peptidyl-histidine |
Full defintion | The modification of peptidyl-histidine to 2'-(3-carboxamido-3-(trimethylammonio)propyl)-L-histidine, known as diphthamide, found in translation elongation factor EF-2. The process occurs in eukaryotes and archaea but not eubacteria. |
Subterm of |
The relationship of GO:0017183 with other GO terms.
Relationship type | GO terms |
---|---|
Is a | |
Regulates | n.a. |
Part of | n.a. |
Positively regulates | n.a. |
Negatively regulates | n.a. |
A force layout showing the ancestor tree for GO:0017183, and its immediate children. If you wish to explore the tree dynamically, please use the GO Explorer.
This table contains additional metadata associated with the GO entry's definition field.
Field | Value |
---|---|
GOC | pde |
RESID | AA0040 |
PMID | Diphthamide biosynthesis requires an organic radical generated by an iron-sulphur enzyme. Nature. 2010 Jun 17; 465 (7300): 891–6.PMID: 20559380 Archaeal and eukaryotic translation elongation factor 2 contain a unique post-translationally modified histidine residue called diphthamide, which is the target of diphtheria toxin. The biosynthesis of diphthamide was proposed to involve three steps, with the first being the formation of a C-C bond between the histidine residue and the 3-amino-3-carboxypropyl group of S-adenosyl-l-methionine (SAM). However, further details of the biosynthesis remain unknown. Here we present structural and biochemical evidence showing that the first step of diphthamide biosynthesis in the archaeon Pyrococcus horikoshii uses a novel iron-sulphur-cluster enzyme, Dph2. Dph2 is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. Biochemical data suggest that unlike the enzymes in the radical SAM superfamily, Dph2 does not form the canonical 5'-deoxyadenosyl radical. Instead, it breaks the C(gamma,Met)-S bond of SAM and generates a 3-amino-3-carboxypropyl radical. Our results suggest that P. horikoshii Dph2 represents a previously unknown, SAM-dependent, [4Fe-4S]-containing enzyme that catalyses unprecedented chemistry. |
GO predictions are based solely on the InterPro-to-GO mappings published by EMBL-EBI, which are in turn based on the mapping of predicted domains to the InterPro dataset. The InterPro-to-GO mapping was last updated on , while the GO metadata was last updated on .
Transcript | Name | Description | GO terms | GO count |
---|---|---|---|---|
– | PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum] gi|502124464|ref|XP_004498540.1| | 1 | ||
– | PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum] gi|502124464|ref|XP_004498540.1| | 1 | ||
– | PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum] gi|502124464|ref|XP_004498540.1| | 1 | ||
– | PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum] gi|502124464|ref|XP_004498540.1| | 1 | ||
– | PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum] gi|502124464|ref|XP_004498540.1| | 1 | ||
– | PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum] gi|502124464|ref|XP_004498540.1| | 1 | ||
– | PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum] gi|502124464|ref|XP_004498540.1| | 1 | ||
– | PREDICTED: probable diphthine synthase-like [Glycine max] gi|356521535|ref|XP_003529410.1| | 2 | ||
– | PREDICTED: probable diphthine synthase-like [Glycine max] gi|356521535|ref|XP_003529410.1| | 2 | ||
– | Diphthine synthase-like protein; TAIR: AT4G31790.1 Tetrapyrrole (Corrin/Porphyrin) Methylase; Swiss-Prot: sp|O81769|DPH5_ARATH Probable diphthine methyl ester synthase; TrEMBL-Plants: tr|A0A0R4J642|A0A0R4J642_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0428200 | 2 | ||
– | Diphthine synthase-like protein; TAIR: AT4G31790.1 Tetrapyrrole (Corrin/Porphyrin) Methylase; Swiss-Prot: sp|O81769|DPH5_ARATH Probable diphthine methyl ester synthase; TrEMBL-Plants: tr|A0A0R4J642|A0A0R4J642_SOYBN Uncharacterized protein; Found in the gene: LotjaGi1g1v0428200 | 2 | ||
– | Diphthamide biosynthesis protein; TAIR: AT3G59630.1 diphthamide synthesis DPH2 family protein; Swiss-Prot: sp|A4QN59|DPH2_DANRE Diphthamide biosynthesis protein 2; TrEMBL-Plants: tr|A0A0S3SFB0|A0A0S3SFB0_PHAAN Uncharacterized protein; Found in the gene: LotjaGi1g1v0575100 | 1 | ||
– | Diphthamide biosynthesis protein; TAIR: AT5G62030.1 diphthamide synthesis DPH2 family protein; Swiss-Prot: sp|Q54PW5|DPH1_DICDI Diphthamide biosynthesis protein 1; TrEMBL-Plants: tr|G7K6J3|G7K6J3_MEDTR Diphthamide biosynthesis protein; Found in the gene: LotjaGi2g1v0448400 | 1 | ||
– | Diphthamide biosynthesis protein; TAIR: AT5G62030.1 diphthamide synthesis DPH2 family protein; Swiss-Prot: sp|Q54PW5|DPH1_DICDI Diphthamide biosynthesis protein 1; TrEMBL-Plants: tr|G7K6J3|G7K6J3_MEDTR Diphthamide biosynthesis protein; Found in the gene: LotjaGi2g1v0448400 | 1 | ||
– | Diphthine synthase-like protein; TAIR: AT4G31790.1 Tetrapyrrole (Corrin/Porphyrin) Methylase; Swiss-Prot: sp|O81769|DPH5_ARATH Probable diphthine methyl ester synthase; TrEMBL-Plants: tr|A0A0R0JDS4|A0A0R0JDS4_SOYBN Uncharacterized protein; Found in the gene: LotjaGi5g1v0272000 | 2 |
A list of co-occurring GO terms within the L. japonicus gene space:
GO term | Namespace | Name | Observations | Saturation (%) |
---|---|---|---|---|
Biological process | Peptidyl-diphthamide biosynthetic process from peptidyl-histidine | 1 | 6.67 |